strains of trichoderma harzianum s. l. have a great importance because of affecting mankind by being used as biological control agents against plant pathogens. in the present study, we planned to characterize the phylogenetic structure of t. harzianum recovered from different geographical origins and various habitats in some climates of iran (nc, scd, csw). in order to screen 239 collected tric...

recent advances in dna based technology have made available a wide range of molecular characteristics for taxonomic and systematic studies of malaria vectors. one region of the anopheline mosquito genome that has received particular attention is the ribosomal dna. in this study, mosquitoes of the anopheles superpictus collected from a wide geographical distribution of iran were investigated for...

for study the genetic diversity and phylogenetic analysis of polymyxa betae keskin , isolated from sugar beet fields in razavi and northern khorasan provinces ,soil of 49 fields were sampled and sugar beet seeds (variety ic) were planted that .after dna extraction, a genomic region comprises of 463 bp of p.betae isolation including 18s r rna، its1 ,5.8srrna ،its2 and 28s rrna was amplified usin...

BACKGROUND The radix of Glehnia littoralis Fr. Schmidt ex Miq. (Beishashen), is often misidentified and adultered in Chinese medicine. Its seven common adulterants include Chuanminshen violaceum Sheh et Shan (Chuanmingshen), Changium smyrnioides Wolff (Mingdangshen), Sphallerocarpus gracilis (Bess.) K.-Pol. (Miguoqin), Adenophora polyantha Nakai (Shishashen), Silene tatarinowii Regel (Shishengy...

The ITS2 gene class shows a high sequence divergence among its members that have complicated its annotation and its use for reconstructing phylogenies at a higher taxonomical level (beyond species and genus). Several alignment strategies have been implemented to improve the ITS2 annotation quality and its use for phylogenetic inferences. Although, alignment based methods have been exploited to ...

Due to difficulties concerning morphological identification of planorbid snails of the genus Biomphalaria, and given a high variation of characters and in the organs with muscular tissue, we designed specific polymerase chain reaction (PCR) primers for Brazilian snail hosts of Schistosoma mansoni from available sequences of internal transcribed spacer 2 (ITS2) of the ribosomal RNA gene. From th...

BACKGROUND Internal transcribed spacer of nuclear ribosomal DNA (nrDNA) is already one of the most popular phylogenetic and DNA barcoding markers. However, the existence of its multiple copies has complicated such usage and a detailed characterization of intra-genomic variations is critical to address such concerns. METHODOLOGY/PRINCIPAL FINDINGS In this study, we used sequence-tagged pyroseq...

Internal transcribed spacer region (ITS) sequencing is the most extensively used technology for accurate molecular identification of fungal pathogens in clinical microbiology laboratories. Intra-genomic ITS sequence heterogeneity, which makes fungal identification based on direct sequencing of PCR products difficult, has rarely been reported in pathogenic fungi. During the process of performing...

background: infectivity of herbivores with trichostrongylus nematodes is widespread in many countries, having a major economic impact on breeding, survivability, and productivity of domestic livestock. this study was carried out on trichostrongylus species isolated from domestic livestock in order to develop an easy-to-perform method for species identification. methods: trichostrongylus isolate...

Invasive fungal disease often plays an important role in the morbidity and mortality of immunocompromised patients. The poor sensitivity of current fungal blood culture and histological practices has led to the development of highly sensitive and specific molecular techniques, such as the PCR. Sequence variability of the internal transcribed spacer 2 (ITS2) region of fungi is potentially useful...