the interaction between isatin-β-thiosemicarbazone (ibt) and calf thymus dna (ct-dna) was investigated in physiological buffer (ph 7.4) using neutral red (nr) dye as a spectral probe by uv–vis absorption and fluorescence spectroscopy, as well as viscosity measurements. the ibt is stabilized by intercalation in the dna ( k [ibt –dna] =1.03×105 m−1), and displaces the nr dye from the nr–dna comple...

A media consisting of isatin-Schiff bases and its inclusion complexes with β-cyclodextrine was developed to maximize the production of antibiotics Hexaene H-85 and azalomycine B by Streptomyces hygroscopicus CH-7. The media with β-cyclodextrine inclusion complex of isatin-3-thiosemicarbazone resulted in the maximum antibiotics concentration of 493 μg/cm for Hexaene H-85 and 191 μg/cm for azalom...

The RNA-dependent DNA polymerase of Rous sarcoma virus is inhibited by N-methyl isatin beta-thiosemicarbazone and by thiosemicarbazide, but not by semicarbazide. These inhibitors also inactivate, upon contact with the virion, the transforming ability of Rous sarcoma virus. Sulfhydryl donors, such as 2-mercapto-ethanol, can prevent these effects. The RNA-directed activity of the purified polymer...

The composition and stability of the complexes of isatin-β-thiosemicarbazone with Co(II), Ni(II), Cu(II), Zn(II), Pd(II) and Hg(II) have been investigated using spectrophotometric method at 30 °C and constant ionic strength of 0.1 mol dm-3 (KNO3) in 70 % ethanol. Experimental results indicate the formation of MeL and MeL2 complexes for Ni(II) and Co(II), and MeL for Cu(II), Zn(II), Pd(II) and H...

The minimal inhibitory concentrations of various antibiotics for a strain of Streptococcus pyogenes and a stable L-form of it were determined. These results were evaluated with those of other investigators similarly performed with S. faecalis and derived protoplasts. Differences in reaction to puromycin, bacitracin and ristocetin were apparent only between the wall-less derivatives. The polyene...

Herpes simplex and vaccinia viruses and adenovirus types 1, 2, 5, and 7 were tested by plaque suppression methods for sensitivity to halogenated deoxyuridines (5-iodo-, 5-bromo-, 5-chloro-, and 5-fluoro-), cytosine arabinoside, isatin-beta-thiosemicarbazone, and N-methylisatin-beta-thiosemicarbazone. After incubation for 12 days in HeLa cell cultures, vaccinia virus plaques were still readily s...

The interaction between isatin-β-thiosemicarbazone (IBT) and calf thymus DNA (CT-DNA) was investigated in physiological buffer (pH 7.4) using Neutral Red (NR) dye as a spectral probe by UV-Vis absorption and fluorescence spectroscopy, as well as viscosity measurements. The IBT is stabilized by intercalation in the DNA (K [IBT -DNA] = 1.03×10(5) M(-1)), and displaces the NR dye from the NR-DNA co...

The interaction between isatin-β-thiosemicarbazone (IBT) and calf thymus DNA (CT-DNA) was investigated in physiological buffer (pH 7.4) using Neutral Red (NR) dye as a spectral probe by UV–Vis absorption and fluorescence spectroscopy, as well as viscosity measurements. The IBT is stabilized by intercalation in the DNA ( K [IBT –DNA] =1.03×105 M−1), and displaces the NR dye from the NR–DNA comple...