theplant pathogenic phytophthora drechsleri is morphologically similar to some other non-papillate phytophthora spp., especially p. melonis, and it is difficult to discriminate these convergent taxa. it seems that the iranian putative p. drechsleri isolates from different cucurbit species have been generally misidentified and their characteristics do not match with p. drechsleri. in order to co...

abstract- desert truffles are hypogeous ascomyceteous ectomycorrhizal fungi, occurring in arid and semi-arid ecosystems. a pcr-based method was developed for the identification of 3 major desert truffles of iran: terfezia claveryi, tirmania pinoyi and tirmania nivea based on internal transcribed spacers of rdna. two specific pcr primers were designed for t. claveryi, 4 for t. pinoyi, and 2 for ...

dunaliella is a green halotolerant microalga, which has several industrial applications e.g. β-carotene production. identification of different dunaliella species has been carried out by morpho-physiological and recently molecular studies. to achieve an improved understanding of taxonomy, these studies are required to be in linkage. the present study describes molecular and specific morpho-phys...

objective(s) in addition to several molecular methods and in particular 16s rdna analysis, the application of a more discriminatory genetic marker, i.e., 16s-23s internal transcribed spacer gene sequence has had a great impact on identification and classification of mycobacteria. in the current study we aimed to apply this sequencing power to conclusive identification of some iranian clinical s...

Objective(s) In addition to several molecular methods and in particular 16S rDNA analysis, the application of a more discriminatory genetic marker, i.e., 16S-23S internal transcribed spacer gene sequence has had a great impact on identification and classification of mycobacteria. In the current study we aimed to apply this sequencing power to conclusive identification of some Iranian clinical ...

Eco-RI-A fragments of the human ribosomal RNA gene family from two types of tissue and three individuals were cloned in lambda vectors and compared by restriction enzyme digestion and electron microscopy. The EcoRI fragment A contains (i) 0.2 kb of the 3' end of the 18S rDNA, (ii) 2.5 kb of internal transcribed spacer and the 5.8S rDNA, and (iii) 4.6 kb of the 28S rDNA gene. All of the six clon...

during spring and summer of 2012, a disease characterized by panicle blight was observed on some pistachio (pistacia vera l.) trees grown in kerman province. eighteen isolates of a botryosphaeriaceae were obtained from affected panicles. based on morphological and molecular characteristics, isolates were identified as botrysphaeria dothidea. to our knowledge, this is the first report of b. doth...

The alignment of the 28S gene of several species of Pezizales allowed to select two pairs of primers able to amplify the internal transcribed spacer region of ribosomal DNA in mycorrhizal fungi, such as truffles. The higher yield of the amplification product demonstrates a better annealing of the new primers to the rDNA, as compared to the universal primers internal transcribed spacer 1 and int...

background: accurate identification of sheep nematodes is a critical point in epidemiological studies and monitoring of drug resistance in flocks. however, due to a close morphological similarity between the eggs and larval stages of many of these nematodes,such identification is not a trivial task. there are a number of studies showing that molecular targets in ribosomal dna (internal transcri...

a specimen of an ascomycetous fungus collected during a field trip was identified as gymnoascus baran. soil samples collected from 0-20 cm depth were studied for isolation of fungi using a soil dilution plate method. based on the morphological and molecular characters the specimens were identified as gymnoascus reesii. genomic dna was extracted and a nuclear rdna region, containing the internal...