background: leukemia inhibitory factor (lif) is a 45-56 kda glycoprotein that has an important role in proliferation and embryo implantation. its effect on oocyte maturation and how to exert the function remained to be elucidated. methods: immature mice superovulated with human menopausal gonadotropin and germinal vesicle (gv) oocytes were obtained from ovary 48 hours after. gv oocytes were cul...

in vitro maturation (ivm) is a method that immature oocytes in antral follicles are extracted and matured in laboratry conditions. this review has attempted to provide the current knowledge and recent findings in in vitro maturation of oocytes and highlights the most important factors involved in this process. the review is based on literature reports and the author’s experience. in ivm cycles,...

objective: crocin is an active ingredient of saffron (crocus sativus l.) and its antioxidant properties have been previously investigated. this carotenoid scavenges free radicals and stimulates glutathione (gsh) synthesis; consequently, it may protect cells against oxidative stress. the aim of this research is to protect oocytes from oxidative stress by the addition of a natural source antioxid...

Background: The pregnancy rates from oocytes matured in vitro are much lower than those of in vivo stimulation cycles, indicating that optimization of IVM remains a challenge. Reduced developmental competence of oocytes matured in vitro indicates that current culture systems for oocytes maturation do not adequately support nuclear and/or cytoplasmic maturation. Therefore in this study, we evalu...

background:   objective: in this study, we supplemented a chemically defined medium with different combinations of selenium, calcium and calcium ionophore concentrations to obtain the best rate of human oocytes maturation, survival, and fertilization.   materials and methods: as an experimental study, three combinations of [selenium (5 μg/ml), calcium (5 μg/ml) and calcium ionophore (1 μg/ml)],...

abstract: introduction & objective: the infertility treatment by using in vitro matured oocyte has many potential applications. it minimizes the risk of ovarian hyper stimulation and is an alternative treatment for women with polycystic ovarian syndrome who may have problems regarding stimulation for ivf. this method may prove important for subjects in need of fertility preservation and also pr...

Background: The use of C-type natriuretic peptide (CNP) combined with cysteamine during pre-in vitro maturation (IVM) can help establish an effective pre-IVM system. Aims: This study was designed to investigate the effects of CNP combined with cysteamine on meiotic maturation and developmental competence of bovine oocytes in vitro. Met...

introduction: superovulation produces complications in some patients, so invitro maturation of oocytes is used to decrease or eliminate these complications and improve ivf. moreover, ivm is used for different aspects of reproductive researches. slaughterhouse ovaries are the main source of oocytes for ivm and ivf studies. different media has been introduced and experimented for in vitro maturat...

Background: In vitro maturation (IVM) is a promising treatment option for certain infertile women. Nowadays, with the aid of the Polscope, it has become possible to evaluate zona pellucida (ZP) and spindle as parameters of oocyte quality. The goal was to investigate the relationship between the presence of the meiotic spindle and ZP birefringence with morphology of the in vivo and in vitro matu...