Genome uncoating is a prerequisite for the successful infection of plant viruses in host plants. Thus far, little is known about the genome uncoating of the Carnation mottle virus (CarMV). Here, we obtained two reconstructions of CarMV at pH7 in the presence (Ca-pH7) and absence (EDTA-pH7) of calcium ions by Cryo-EM single particle analysis, which achieved 6.4 Å and 8 Å resolutions respectively...

Monoclonal antibody PH7 has specificity for the phosphorylated form of the human liver phenylalanine hydroxylase and negligible reactivity towards the dephosphorylated form of the native enzyme by enzyme-linked immunoassay. PH7 binds specifically to the phosphorylated form of the liver enzyme after SDS/polyacrylamide-gel electrophoresis and transfer to nitrocellulose. Competitive blocking assay...

Both rat liver and Escherichia coli rRNA in 0.1M-sodium chloride were titrated with acid or alkali over the range pH 3-7 at approx. 0°C. rRNA did not bind acid reversibly and hysteresis was observed, i.e. the plot of acid bound to rRNA against pH had the form of a loop showing that the amount of acid bound at a particular pH depended on the direction of the titration. Although the boundary curv...

The present study aimed to evaluate possible variations in the chemical composition and antimicrobial activity of essential oils extracted from Piper marginatum leaves obtained by hydrodistillation at pH4, pH7, pH10. When compared, profiles three were different. A reduction major phenylpropanoids Z-asarone, dillapiole, E-asarone was observed for samples pH4-EO pH10-EO as compared sample pH7- EO...

1. Intracellular recording techniques were used to study the effects of temperature on resting membrane conductances, electrical excitability and synaptic efficacy in fast-glycolytic (FG) skeletal muscle fibres from the lizard Dipsosaurus dorsalis. 2. The conductance of the resting muscle membrane to chloride ions (gci) increased from 488/iScm" at 15°C (pH7-8) to 730/iScm~ at 45°C (pH7-4), yiel...

The latency of nitrocatechol sulphatase activity was measured in rat liver lysosomes before and after preincubation in 0.25m solutions of five amino acids, three dipeptides and a tripeptide. Rates of increase in ;free' activity were taken as an indication of rates of solute penetration into lysosomes and were correlated with the structure of each molecule studied. In general permeability was gr...

Heat-induced plant protein gels commonly require high concentration and temperatures. However, hydrolysis of some proteins may promote their gelation under milder conditions. In this study, Alcalase (EC No. 3.4.21.62) was used to quickly (3 min) hydrolyze pea proteins, the physicochemical properties gelling capacity hydrolysates were investigated. Hydrolysates showed a degree more than 6.5%, mo...

1. A new method has been developed for the specific extraction of histone fraction f2(a) from calfthymus deoxyribonucleoprotein at pH 7 by using a mixture of ethanol and guanidinium chloride. 2. Fraction f2(a) has been separated into the subfractions f2(a)1 and f2(a)2 by acetone precipitation from acid solution, and at pH7. 3. Modifications of existing electrophoretic methods are described that...

for the enzyme in vivo unless an alkaline micro-environment was postulated or conditions were present in vivo which could potentiate inhibition by UMP at near-neutral pH (Yon, 19726). Substances such as deoxycholate which can bind to the hydrophobic site on the enzyme may provide such potentiation. Fig. 2 shows the effects of UMP and deoxycholate at pH values between 7 and 11. In the absence of...