in order to reduce the costs associated with poly-β-hydroxybutyrate production, growth and poly-β-hydroxybutyrate production of ralstonia eutropha were studied in batch culture on different carbon sources. experiments were designed and conducted to not only lower the cost of poly-β-hydroxybutyrate production by using inexpensive substrates, but also to increase poly-β-hydroxybutyrate production...

conclusions the results suggest that b. coagulans is a good candidate for the fermentative production of phb. background biodegradable polyesters are candidates for the development of environmental friendly plastics. poly-β-hydroxybutyrate (phb) is a type of polyester from the hydroxyalkanoates family, synthesized by bacteria as an intracellular material and accumulated as granules in the cytop...

Exogenous 3-hydroxybutyrate can be utilized by a variety of soil bacteria as a carbon and energy source. However, the membrane transporter responsible for 3-hydroxybutyrate uptake remains unidentified. The Bacillus subtilis strain 168 gene yxjC (herein renamed hbuT) encodes a putative gluconate transporter GntT-type membrane transporter with a previously unknown function. hbuT is organized with...

OBJECTIVES To evaluate performance characteristics of the newly available handheld combined glucose and ketone meter for beta-hydroxybutyrate measurement. DESIGN Laboratory method evaluation. MAIN OUTCOME MEASURES Accuracy of beta-hydroxybutyrate measurement and effect of acetoacetate interference at clinically important beta-hydroxybutyrate levels. RESULTS Deming regression analysis of b...

A recombinant Escherichia coli strain has been developed that produces poly(3-hydroxybutyrate-co-4-hydroxybutyrate) when grown in complex medium containing glucose. This has been accomplished by introducing into E. coli DH5 alpha separate plasmids harboring the polyhydroxyalkanoate (PHA) biosynthesis genes from Ralstonia eutropha (formerly named Alcaligenes eutrophus) and the succinate degradat...

background and objective: production of polyhydroxyalkanoate copolyesters consisting of 3-hydroxybutyrate and 3-hydroxyvalerate units was for the first time studied using the production strain hydrogenophaga pseudoflava based on sustainable raw materials. this strategy provides for increased cost efficiency in pha production and in enhanced material quality. material and methods: as a particula...

3-Hydroxypropionate (3HP) is an important compound in the chemical industry, and the polymerized 3HP can be used as a bioplastic. In this review, we focus on polyesters consisting of 3HP monomers, including the homopolyester poly(3-hydroxypropionate) and copolyesters poly(3-hydroxybutyrate-co-3-hydroxypropionate), poly(3-hydroxypropionate-co-3-hydroxybutyrate-co-3-hydroxyhexanoate-co-3-hydroxyo...

INTRODUCTION Gamma-hydroxybutyrate is a potent sedative-hypnotic agent and a popular drug of abuse. In the United States, gamma-hydroxybutyrate is a Schedule I controlled substance (sodium oxybate) with orphan drug status for the treatment of narcolepsy within approved clinical studies. Physostigmine is a carbamate inhibitor of acetylcholinesterase that is reported to attenuate the sedative eff...