background: there are varieties of purification techniques for separation of human plasma proteins such as salting out, ion exchange chromatography, and ethanol fractionation. there are limitations for each method, for example in salting out method, the salt has to be removed in an additional step. ion exchange chromatography is difficult for scaling up, and plasma fractionation is a time consu...

mammalians express several subclasses of the igg molecule. in human being there are four homologous igg subclasses, each of which is structurally unique and has different functions. quantification of igg subclasses is fundamental to clinical assessment and diagnosis of many diseases as such assessments depends on the availability of subclassspecific antibodies (abs), particularly monoclonal ant...

host genetic factors play a central role in determining the clinical phenotype of human diseases. association between two polymorphic loci in human genome, human leukocyte antigen (hla) and killer cell immunoglobulin-like receptors (kirs), and genetically complex infectious disease, particularly those of viral etiology, have been historically elusive. hence, defining the influence of genetic di...

human parvovirus b19 is a very common viral infection especially in school-aged children. the infection during pregnancy can affect the fetus due to lack of mother's immunity. although, there is still no evidence of fetal teratogenic effects with parvovirus b19, but non-immune fetal hydrops and abortion may be caused by vertical transmission of the virus during pregnancy. this study was aimed t...

measles is one of the most contagious human diseases. although mass vaccination programs have reduced the incidence of this disease, measles is still an important cause of morbidity and mortality among children in developing countries. therefore the use of sensitive techniques to evaluate vaccine efficacy and level of immunity among members of susceptible communities is crucial. serum neutraliz...

background: pediatric bronchial asthma is associated with considerable morbidity. the study was carried out to examine the association of human leukocyte antigen (hla)- class ii with the disease as we found no similar study on asian indian population. objective: to define the hla-class ii antigens in asian indian pediatric patients with asthma. methods: a total of 103 children with asthma and 1...

As IgM and IgA-enriched preparations are needed to complete the immunotherapeutic spectrum, a simple procedure is described for the preparation of IgM and IgA enriched immunoglobulins. Fraction III which was prepared by cold ethanol fractionation was treated by octanoic acid followed by ethanol precipitation and ion-exchange chromatography using Sephadex DEAE A-50 and 0.1 M tris-D.35M NaCI ...

immunoglobulin e is one of the five classes of immonoglobulins that plays an important role in allergic diseases. production of monoclonal antibodies by a single clonotype against different epitopes of immunoglobulin e has high priority in development of diagnostic kits. in this study, an attempt was made to produce monoclonal antibodies against human immunoglobulin e. balb/c mice were immunize...