background: the radioprotective effect of grewia asiatica fruit (gae) which contains anthocyanin type cyanidin 3- glucoside, vitamin c, a, minerals, carotenes and dietary fibers etc was studied. materials and methods: for study swiss albino mice were divided into five groups-1. control (vehicle treated) 2. gae treated (700 mg / kg. b.wt / day for fifteen days), 3. irradiated (5 gy), 4. gae + ir...

high levels of regulated oncogen-alpha (gro-a) expression have been observed in the liver. gro-a stimulates proliferation of epithelial cells and induction of rolling and extravascular migration of neutrophils and mononuclear cells. given the above observations, this chemokine was chosen to be analyzed in freshly isolated and cultured hepatocytes. in this study, hepatocytes (2×106 cell/ml) were...

Background and Aims: Human adipose tissue-derived stem cells (hASCs) are considered as an attractive source of regenerative stem cells, mainly because of their higher proliferation rate, more accessibility and hepatocyte like properties as compared to mesenchymal stem cells isolated from other tissues. Numerous studies have described the beneficial use of adipose tissue-derived stem cells for g...

conclusions hepatocyte isolation can be performed as the main and initial step for cell transplantation from unused/rejected liver. it is the first experience in iran. results highly quality hepatocytes with high viability and low contamination were isolated. the mean viability was 71.8% ± 21.7. in the first 4 cases microbial contamination by staphylococci, diphtheroid and klebsiella was detect...

stromal derived factor-a (sdf-1-a) is a cxc chemokine which has been demonstrated as a recruitment factor for leukocytes to the site of inflammation, infection, injury and following stress. this chemokine has been shown to be expressed by liver cells and in liver diseases. hence, the aim of this study was to examine the expression of sdf-1 by hepatocytes in responses to the stress imposed durin...

SUMMARY One intraperitoneal injection of 1 mg of 3-methylchol anthrene into immature male and female rats results in an increase in azo dye demethylase activity. This is followed by an increase in wet and dry liver weight and in total protein, which reaches a peak of about 30†" 40% above control levels within 3 or 4 days. Cell proliferation contributes to the in crease in liver weight and pro...

in this present study, we examined the differentiation potential of human bone marrow derived mesenchymal stem cells (hbmscs) into hepatocytes on a three-dimentional (3d) nanofibrous scaffold formed by poly (ε-caprolactone) (pcl), collagen and polyethersulfone (pes). the nanofiber was prepared by the electrospining technique. hbmscs were isolated using combining gradient density centrifugation ...

The growth characteristics of Tyzzer's organisms in cultured mouse hepatocytes were examined by direct bacterial counting and plaque assay. The viability of propagated bacteria and time required for the maximum titers depended on incubation time and inoculum dose, respectively. Both infectious titers and bacterial counts at optimum harvests were much higher than those obtained from infected mou...

isolated rat hepatocytes in culture were incubated with different concentrations of iron-sorbitol (50, 100, 150, and 200 µm) to assess the changes in reactive oxygen species (ros) and lipid peroxidation leading to apoptotic hepatocyte cell death. the viability of hepatocytes was declined depending on the iron concentration. one hour incubation of the cells with 100 µm iron resulted in decreased...