background: maturity-onset diabetes of the young (mody) is a heterogeneous entity of monogenic disorders characterized by autosomal dominant inheritance. eleven genes were related, including hnf4α, gck, hnf1α, ipf1, and hnf-1β, and various mutations are being reported. methods: to help the overall understanding of mody-related pathologic mutations, we studied a large mody family found in 2012, ...

Liver organogenesis starts around day 10 of mouse embryonic development and is completed after birth. This process requires the simultaneous differentiation of several cell types, including hepatocytes and biliary epithelial cells (BEC), and the formation of precise three-dimensional structures. Several groups have attempted to unravel the genetic program that controls hepatocytes and BEC diffe...

There is a lack of current treatment options for ovarian clear cell carcinoma (CCC) and the cancer is often resistant to platinum-based chemotherapy. Hence there is an urgent need for novel therapeutics. The transcription factor hepatocyte nuclear factor 1β (HNF1β) is ubiquitously overexpressed in CCC and is seen as an attractive therapeutic target. This was validated through shRNA-mediated kno...

CONTEXT Heterozygous mutations or deletions of the transcription factor hepatocyte nuclear factor 1β (HNF1β) result in a heterogeneous syndrome characterized by renal cysts and diabetes, together with a variety of other extrarenal and renal manifestations. Interestingly, in several patients with HNF1β abnormalities, we observed early hyperparathyroidism and PTH levels that we judged inappropria...

Bookmarking factors are transcriptional regulators involved in the mitotic transmission of epigenetic information via their ability to remain associated with mitotic chromatin. The mechanisms through which bookmarking factors bind to mitotic chromatin remain poorly understood. HNF1β is a bookmarking transcription factor that is frequently mutated in patients suffering from renal multicystic dys...

Hepatocyte nuclear factor 1β (HNF1β) is an essential transcription in development of the kidney, liver, and pancreas. HNF1β-mediated target genes dependent on cell type stage. Nevertheless, regulation HNF1β function by enhancers co-factors that allow this cell-specific largely unknown. To map interactome we performed mass spectrometry a mouse kidney inner medullary collecting duct line. Pterin-...

NG2-expressing cells are a population of periportal vascular stem/progenitors (MLpvNG2(+) cells) that were isolated from healthy adult mouse liver by using a "Percoll-Plate-Wait" procedure. We demonstrated that isolated cells are able to restore liver function after transplantation into a cirrhotic liver, and co-localized with the pericyte marker (immunohistochemistry: PDGFR-β) and CK19. Cells ...

objective(s):fuzheng huayu recipe (fzhy) exerts significant protective effects against liver fibrosis by strengthening the body’s resistance and removing blood stasis. however, the molecular mechanisms through which fzhy affects liver fibrosis are still unclear. in this study, we examined the expression levels of factors involved in the inhibitor κb kinase-β (ikk-β)/nuclear factor-κb (nf-κb) an...

Objective(s):Fuzheng Huayu recipe (FZHY) exerts significant protective effects against liver fibrosis by strengthening the body’s resistance and removing blood stasis. However, the molecular mechanisms through which FZHY affects liver fibrosis are still unclear. In this study, we examined the expression levels of factors involved in the inhibitor κB kinase-β (IKK-β)/nuclear factor-κB (NF-κB) an...

objective: micrornas (mirnas) are noncoding rnas that function as key regulators of diverse biological activities such as cellular metabolism, cell proliferation and cell cycle regulation. recent studies have indicated the high potential of these small molecules to control stem cell differentiation into desired cells. the aim of present study is to investigate the possible effect of let-7f on e...