objective(s): we investigated the contribution of gyra and parc mutational mechanism in decreased ciprofloxacin susceptibility of acinetobacter baumannii isolated from burn wound infections. materials and methods: ciprofloxacin susceptibility of 50 a. baumannii isolates was evaluated by disk diffusion and agar dilution methods. pcr and sequencing were performed for detection of mutation in gyra...

Objective(s): We investigated the contribution of gyrA and parC mutational mechanism in decreased ciprofloxacin susceptibility of Acinetobacter baumannii isolated from burn wound infections. Materials and Methods: Ciprofloxacin susceptibility of 50 A. baumannii isolates was evaluated by disk diffusion and agar dilution methods. PCR and sequencing were performed for detection of mutation in gyr...

background: emergence klebsiella pneumoniae resistant to quinolone antibiotics due to mutations in gyra and parc genes created problem for treatment of patients in different hospitals in iran. the objective of this study was to determine the amino acid substitutions of gyra and parc proteins in certain clonal lineages of the k. pneumoniae conferring high level quinolone resistance. methods: one...

Background: Emergence Klebsiella pneumoniae resistant to quinolone antibiotics due to mutations in gyrA and parC genes created problem for treatment of patients in different hospitals in Iran. The objective of this study was to determine the amino acid substitutions of GyrA and ParC proteins in certain clonal lineages of the K. pneumoniae conferring high level quinolone resistance. Methods: One...

Type II topoisomerases, including DNA gyrase (GyrA) and topoisomerase IV (ParC), contribute to fluoroquinolone resistance in Enterococcus spp. This study investigated the mutational status of quinolone resistance-determining regions (QRDRs) GyrA ParC clinical isolates enterococci from a hospital Baotou, China. We analyzed 110 enterococcal isolates, 57 faecalis 53 faecium. The rates E. faecium c...

strain with no parC mutations. This is consistent with previous findings that gyrA mutations are necessary for quinolone resistance, whereas parC mutations appear to be complementary, as resistant strains do not carry parC mutations alone. – 6 Some studies further suggest that additional parC mutations facilitate increasing levels of resistance, but our study did not support this. For example, ...

OBJECTIVES We investigated the contribution of gyrA and parC mutational mechanism in decreased ciprofloxacin susceptibility of Acinetobacter baumannii isolated from burn wound infections. MATERIALS AND METHODS Ciprofloxacin susceptibility of 50 A. baumannii isolates was evaluated by disk diffusion and agar dilution methods. PCR and sequencing were performed for detection of mutation in gyrA a...

Mutations in the quinolone resistance-determining regions (QRDR) in chromosomal gyrA and parC genes and fluoroquinolone susceptibility profiles were investigated in quinolone-resistant Enterobacteriaceae isolated from community and hospitalized patients in the Brazilian Southeast region. A total of 112 nalidixic acid-resistant enterobacterial isolates collected from 2000 to 2005 were investigat...

We investigated the contribution of quinolone resistance-determining region (QRDR) mutations to fluoroquinolone (enrofloxacin, orbifloxacin and danofloxacin) susceptibility in 58 Mycoplasma bovis isolates from dairy cattle in Japan. Fluoroquinolone non-resistant isolates (fluoroquinolone-MICs≤2 μg/ml) possessed no QRDR mutations (19 isolates) or Ser83Leu in GyrA (32 isolates). Fluoroquinolone-r...