Objective(s): Regarding the global burden of uropathogenic Escherichia coli (UPEC) infections, prevention and treatment of such infections play a significant role in healthcare management. The inordinate use of fluoroquinolones led to a worldwide spread of quinolone-resistant strains. Therefore, this study aimed to investigate mutations in codons 83 and 106 of gyrA gene in UPEC isolates in the ...

HIGHLIGHTS Mutational analysis of antibiotic resistant genes E. coli in UTI Patients. These were detected by PCR and sequenced using NGS. isolates positive for GyrA gene, GyrB tetB tetR gene. The novel mutation was gyrB gene at codon554.

background: enterobacteriaceae are a large group of bacteria widely distributed in nature. escherichia coli is the most com­mon cause of urinary tract infection. two amino acid substitutions, in gyra, are commonly responsible for quinolone re­sistance in e. coli . the aim of this study was molecular survey of nalidixic acid resistance e.coli isolated from patients in the codones of 83 and 87 gy...

conclusions this study demonstrated the specificity and sensitivity of the pcr-sscp method for finding mutations in the gyra gene. due to the sensitivity of most isolates to ciprofloxacin, this antibiotic should be considered an appropriate drug for the treatment of related diseases. background during the past several years, nontuberculous mycobacteria (ntm) have been reported as some of the mo...

background: emergence klebsiella pneumoniae resistant to quinolone antibiotics due to mutations in gyra and parc genes created problem for treatment of patients in different hospitals in iran. the objective of this study was to determine the amino acid substitutions of gyra and parc proteins in certain clonal lineages of the k. pneumoniae conferring high level quinolone resistance. methods: one...

quinolones are a large and widely consumed class of synthetic drugs. expanded-spectrum quinolones, like ciprofloxacin are highly effective against gram-negative bacteria, especially escherichia coli. in e. coli the major target for quinolones is dna gyrase. this enzyme is composed of two subunits, gyra and gyrb encoding by gyra and gyrb, respectively. mutations in either of these genes cause qu...

Background: Emergence Klebsiella pneumoniae resistant to quinolone antibiotics due to mutations in gyrA and parC genes created problem for treatment of patients in different hospitals in Iran. The objective of this study was to determine the amino acid substitutions of GyrA and ParC proteins in certain clonal lineages of the K. pneumoniae conferring high level quinolone resistance. Methods: One...

mara activates two membrane dependent mechanisms of resistance to different antibiotics, such as ciprofloxacin and tetracycline, including promotion of outflux and inhibition of influx of antibiotics. thus, mara causes multiple antibiotic resistance phenotype. the activation of these mechanisms needs overexpression of mara. this could happen through mutation in marr. thus, the aim of this study...