the growth hormone gene could be an attractive candidate gene for milk production in goats. single-strand conformation polymorphism was used to identify polymorphism at the goat growth hormone (ggh) gene. for this purpose, genotyping of 90 talli goat breeds was performed. nine conformational patterns were observed in exon 4 of the ggh gene, with frequencies of 27.7% for the homozygous pattern (...

In this study the genetic polymorphism of growth hormone (GH) gene as a candidate gene in livestock was investigated. Blood samples were randomly collected from 34 Alpine and 42 Saanen goats. DNA was extracted from blood samples and a 365 bp region of exon 5 of the GH genewas amplified by polymerase chain reaction (PCR). PCR products were analyzed using single strand conformation polymorphism (...

Acetyl-coenzyme A carboxylase α (ACC-alpha) is considered as the key regulatory enzyme in fatty acid biosynthesis. ACC-alpha gene is located on Caprine chromosome 11 and is polymorphic in many goat breeds. In the current study, we aimed to find possible single nucleotide polymorphisms (SNPs) in the exon 1 region of the ACC-alpha gene in Iranian Mahabadi goat breed. Genomic DNA was extracted fro...

A glycoprotein (Cpgp40/15)-encoding gene of Cryptosporidium parvum was analyzed to reveal intraspecies polymorphism within C. parvum isolates. Forty-one isolates were collected from different geographical origins (Japan, Italy, and Nepal) and hosts (humans, calves, and a goat). These isolates were characterized by means of DNA sequencing, PCR-restriction fragment length polymorphism (PCR-RFLP),...

The genetic improvement of production traits can be made through marker assisted selection using a candidategene approach. Bone morphogenetic proteins (BMPs) are multifunctional growth factors that belong to thetransforming factor b (TGF-b) superfamily. This study aimed detect the polymorphism BMP-4in different goat breeds by polymerase chain reaction-single strand confirmational (PCR-SSCP) and...

acetyl-coenzyme a carboxylase α (acc-alpha) is considered as the key regulatory enzyme in fatty acid biosynthesis. acc-alpha gene is located on caprine chromosome 11 and is polymorphic in many goat breeds. in the current study, we aimed to find possible single nucleotide polymorphisms (snps) in the exon 1 region of the acc-alpha gene in iranian mahabadi goat breed. genomic dna was extracted fro...

in this study, we investigated the relationship between the genetic polymorphism of growth differentiation factor 9 (gdf9) genes and the litter size in 384 individuals of five breeds of black goats. four pairs of primers were designed to detect single nucleotide polymorphism of gdf9 gene in goats by pcr-sscp. the least square was used to analyze the relation between different genotypes and the ...

in this study the genetic polymorphism of growth hormone (gh) gene as a candidate gene in livestock was investigated. blood samples were randomly collected from 34 alpine and 42 saanen goats. dna was extracted from blood samples and a 365 bp region of exon 5 of the gh genewas amplified by polymerase chain reaction (pcr). pcr products were analyzed using single strand conformation polymorphism (...

The present work was carried out to analyse the κ-casein variants in five Indian goat breeds by SDS-PAGE, PCRRFLP and SSCP method. A total of 152 unrelated blood samples and 102 milk samples belonging to Barbari, Beetal, Marwari, Surti and Local MP (non-descript) goats were used for analysis. SDS-PAGE exhibited κ-casein allele A in all the breeds. Moreover PCR+RFLP analysis also confirmed the p...

The present study suggests the existence of an association between milk production traits and genetic polymorphisms at the growth hormone (GH) gene in the Portuguese indigenous Serrana goat. The DNA from 229 animals of two ecotypes (Jarmelista and Ribatejano) was analysed by polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) technique revealing a high degree of genetic...