the leopard moth, zeuzera pyrina, is a key and worldwide pest damaging walnut as well as many other fruit trees during the larval stages. larvae were collected from infested twigs of walnut trees. midguts from larvae were removed under a stereomicroscope and their homogenates used as the enzyme source. larval midgut extracts showed an optimum activity at ph 8.0, 6.0 and 6.0 for ?-amylase, ?- an...

Three categories of cellulases, endoglucanases, cellobiohydrolases and β-glucosidases, are commonly used in the process of cellulose saccharification. In particular, the activity and characteristics of hyperthermophilic β-glucosidase make it promising in industrial applications of biomass. In this paper, the crystal structure of the hyperthermophilic β-glucosidase from Pyrococcus furiosus (BGLP...

the colorado potato beetle, leptinotarsa decemlineata say (col.: chrysomelidae) is an important pest of potato worldwide and study of its glucosidases is an important step to develop appropriate pest control strategies. in this study, some biochemical aspects of β-glucosidase in the colorado potato beetle were determined. the results showed that β-glucosidase activity in the midgut of adults wa...

the imported willow leaf beetle, plagiodera versicolora is one of the main pests of forest trees worldwide including iran. activities of α- and β-glucosidases in the midgut of p. versicolora adults were studied in this research. the results showed a higher α- glucosidase activity of adultsthan β-glucosidase. the optimal phs for α- and β-glucosidases activities were found to be 6 and 9, respecti...

elm leaf beetle, xanthogaleruca luteola, is a serious pest (particularly at its larval stage) of elm trees in many areas. the existence of three important enzyms namely: ?-amylase, ?- glucosidase and ?-glucosidase in the midgut of the insect at its larval stage was investigated. optimum activity for ?-, ?-glucosidase and ?-amylase was recorded at phs 5.0, 6.0 and 6.0 against p-nitrophenyl-?-d-g...

A gene (bgl) encoding a β-glucosidase in thermophilic actinomycete Thermobifida fusca NTU 22 was cloned into a Yarrowia lipolytica expression system. Heterologous expression resulted in extracellular β-glucosidase production with activity as high as 630 U/mL in a Hinton flask culture filtrate. This recombinant β-glucosidase was purified 9.2-fold from crude culture filtrate by DEAE-Sepharose FF ...

A beta-glucosidase was purified from the digestive fluid of the palm weevil Rhynchophorus palmarum L. (Coleoptera: Curculionidae) by chromatography on anion-exchange, gel filtration, and hydrophobic interaction columns. The preparation was shown to be homogeneous on polyacrylamide gels, beta-glucosidase is a monomeric protein with a molecular weight of 58 kDa based on its mobility in SDS-PAGE a...

We have studied some characteristics of alpha-1,4-glucosidases in human male reproductive organs in order to obtain information on the origin of the enzyme in seminal plasma. Acid and neutral enzymes could be distinguished on the basis of their selective inhibition either by SDS (acid enzyme) or MTT (neutral enzyme). Only the epididymis contained a significant amount of SDS resistant neutral al...

During studies of the nutritional utilization of pyridoxine 5*-b-D-glucoside, a major form of vitamin B6 in plants, we detected two cytosolic b-glucosidases in jejunal mucosa. As expected, one was broad specificity b-glucosidase that hydrolyzed aryl b-D-glycosides but not pyridoxine b-D-glucoside. We also found a previously unknown enzyme, designated pyridoxine-b-D-glucoside hydrolase, that eff...