نتایج جستجو برای: germinal vesicle breakdown (gvbd)
تعداد نتایج: 78726 فیلتر نتایج به سال:
objectives: it was aimed to investigate the effects of different doses of follicle stimulating hormone (fsh) and activin a on the growth and maturation of preantral mouse follicles during the in vitro culture. methods: preantral follicles (90-100 µm in diameter) were harvested from 6-8 week-old syrian mice and cultured in tcm199 culture medium for 6 days to see the effect of fsh and activin a. ...
Background & Aim: In vitro maturation (IVM) of oocyte is a promising technique to reduce the costs and avert the side effects of gonadotropin stimulation for in vitro fertilization (IVF). To better characterize the nature and impact of different hormones and important parameters on the growth and in vitro maintenance of oocyte, the present study was done. The purpose of this study is in vit...
In mammalian females, germ cells remain arrested as primordial follicles. Resumption of meiosis is heralded by germinal vesicle breakdown, condensation of chromosomes, and their eventual alignment on metaphase plates. At the first meiotic division, anaphase-promoting complex/cyclosome associated with Cdc20 (APC/CCdc20) activates separase and thereby destroys cohesion along chromosome arms. Beca...
Two studies were conducted with the overarching goal of determining the extent to which lipolytic changes relate to germinal vesicle breakdown (GVBD) in bovine oocytes matured under thermoneutral or hyperthermic conditions. To this end, cumulus-oocyte complexes underwent in vitro maturation for 0, 2, 4, 6 or 24 h at 38.5 (first study) or 38.5 and 41.0 C (second study; heat stress applied up thr...
Germinal vesicle breakdown (GVBD) of bovine oocytes was completely blocked by cycloheximide added to culture medium at concentrations of 1-20 micrograms/ml. Nevertheless, under such conditions a certain degree of chromatin condensation inside the germinal vesicle was observed. The inhibitory effect was not influenced by the presence or absence of cumulus cells and was fully reversible; but the ...
in order to determine the most effective hormones on the in vitro oocyte maturation (germinal vesicle breakdown - gvbd) in a hybrid sturgeon bester, ovarian follicles were incubated at the migratory nucleus stage in the presence of several steroids, gonadotropin preparations and forskolin. occurrence of gvbd and 17,20β-dihydroxy-4-pregnen-3 -one (dhp) production were followed during an expe...
1. Methylxanthines (IBMX, caffeine, theophylline), which are known inhibitors of c-AMP phosphodiesterase inhibited reversibly progesterone-induced maturation of Xenopus oocyte. 2. The time course of protein phosphorylation during maturation stimulated by progesterone was analysed in Xenopus oocyte : protein phosphorylation increased around germinal vesicle breakdown (GVBD). No quantitative chan...
objective: mitochondria is a critical organelle within the cell and has important role in the oocyte development, thus the aim of this study was the comparison of mitochondrial distribution within the mouse oocyte at germinal vesicle (gv), germinal vesicle breakdown (gvbd) and metaphase ii (mii) stages using laser confocal microscopy (lscm). materials and methods: after superovulation of adult...
In order to determine the most effective hormones on the in vitro oocyte maturation (germinal vesicle breakdown GVBD) in a hybrid sturgeon bester, ovarian follicles were incubated at the migratory nucleus stage in the presence of several steroids, gonadotropin preparations and forskolin. Occurrence of GVBD and 17,20β-dihydroxy-4-pregnen-3 -one (DHP) production were followed during an experiment...
In the brachiopod Terebratalia transversa, each ovarian follicle consists of (i) a prophase-arrested oocyte with an enlarged nucleus (= the germinal vesicle, or GV), and (ii) a surrounding sheath of follicle cells that attach to the oocyte by means of junctional complexes. Within about 80 min after removing a follicle from the ovary, the follicular sheath undergoes a microfilament-mediated retr...
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