this study was conducted to detect polymorphism of gene bmp15, a member of transforming growthfactor β (tgfβ) family which has a crucial role in controlling the ovarian follicles development,ovulation rate and fertility. samples were randomly selected from 91 najdi goats in 3 geographicallocations, northwest, southeast and center of khuzestan province. after dna extraction, amplification of235 ...

background: trastuzumab (herceptin) is a humanized monoclonal antibody (mab) which is used for specific treatment of metastatic breast cancer in patients with overexpression of her2/neu receptor. in this study, we have attempted to develop a biosimilar version of trastuzumab mab. methods: according to in silico studies, the heavy and light chains of trastuzumab mab were designed and constructed...

interferon gamma (ifn-g) is a cytokine produced by activated t lymphocytes and natural killer cells. it plays an important role in innate and adaptive immunity. ifn-g gene is polymorphic and its haplotype has been associated with resistance to nematode infestations which are the most important parasites of domestic sheep worldwide. to evaluate ifn-g polymorphism in iranian shaul sheep populatio...

We took a rather unique approach to investigate the conservation of gene expression of prolamin storage protein genes across two different subfamilies of the Poaceae. We took advantage of oat plants carrying single maize chromosomes in different cultivars, called oat-maize addition (OMA) lines, which permitted us to determine whether regulation of gene expression was conserved between the two s...

Background: Amplification of HER2 is seen in 20-30% of breast cancer cases. Measurement of HER2 gene amplification appears to be of vital importance in planning the treatment schedule for patients with breast carcinoma. The aim of our study was to evaluate HER2 amplification status in malignant and benign breast tumors by differential PCR (dPCR). Materials and Methods: The genomic DNA was ex...

Background: Baculoviral inhibitor of apoptosis repeat-containing 5 (BIRC5) gene is an inhibitor of apoptosis that expresses in human embryonic tissues but it is absent in most healthy adult tissues. The copy number of BIRC5 has been indicated to be highly increased in tumor tissues; however, its association with the age of onset in breast cancer is not well understood. Methods: Forty tumor tiss...

the aim of the current study is to amplification the regularity region encompassed the mutations changing the myostatin gene express and also to determine sequential analysis. the blood samples taken from 12 najdi cattles in najdi cattle station in shushtar city, khuzestan province. then the dna extracted to amplification 730bp and 561bp fragments. sequencing was performed after the precision i...