purpose: to compare clinical and confocal scan outcomes after descemet stripping automated endothelial keratoplasty (dsaek) performed for fuchs’ endothelial dystrophy versus pseudophakic bullous keratopathy. methods: in this retrospective comparative study, 47 consecutive eyes of 39 patients with the diagnosis of fuchs’ endothelial dystrophy (n=29, group 1) or pseudophakic bullous keratopathy (...

purpose: to evaluate endothelial cell density (ecd) and morphology of clear corneal grafts after descemet stripping automated endothelial keratoplasty (dsaek) and to determine the factors that influence graft ecd postoperatively. methods: seventy-seven eyes (38 right eyes) of 64 patients (34 female subjects) who underwent dsaek for fuchs’ endothelial dystrophy (n=38) or pseudophakic bullous ker...

PURPOSE To investigate whether apoptosis plays a notable role in degeneration of corneal endothelial cells in patients with Fuchs' dystrophy. METHODS Forty-seven corneal buttons from 41 patients with Fuchs' dystrophy were studied. Nucleus labeling, transmission electron microscopy (TEM), and TdT-dUTP terminal nick-end labeling (TUNEL) were used to detect apoptosis. TEM and TUNEL were performe...

PURPOSE To describe the morphologic features of Fuchs corneal dystrophy and compare them with those of bullous keratopathy. METHODS This was an observational case series. The study group consisted of 32 corneal buttons with a diagnosis of Fuchs dystrophy and the comparison group consisted of 22 corneal buttons with bullous keratopathy. Morphologic analysis was performed of corneal buttons fro...

OBJECTIVE To investigate the potential role of apoptosis in the pathogenesis of Fuchs endothelial dystrophy of the cornea. METHODS Twenty-one corneal buttons from patients with Fuchs dystrophy and 15 control corneas were studied. Apoptosis was assessed by the in situ end-labeling of double-stranded DNA breaks, and by immunohistochemical characterization of cellular markers associated with apo...

Measurements of corneal thickness, endothelial cell size, endothelial permeability to fluorescein, and intraocular pressure were made in two groups of human subjects: 21 persons with cornea guttata (early Fuchs dystrophy without epithelial edema) and 17 persons age- and sex-matched but with normal corneas. Statistically significant differences were found between the two groups in all four measu...

Citation: Zhang W, Ogando DG, Kim ET, et al. Conditionally immortal Slc4a11 / mouse corneal endothelial cell line recapitulates disrupted glutaminolysis seen in Slc4a11 / mouse model. Invest Ophthalmol Vis Sci. 2017;58:3723–3731. DOI:10.1167/ iovs.17-21781 PURPOSE. To establish conditionally immortal mouse corneal endothelial cell lines with genetically matched Slc4a11þ/þ and Slc4a11 / mice as ...