Identification of the interactions among proteins is crucial to illustrate their functions, and further, can help to understand the biological processes and provide insights into the mechanisms of diseases. It has became one of the most challenging and important task in the postproteomic researches. Comparing with costly and time-consuming biochemical experiments, the computational methods have...

A modified purification is described for an enzyme, from Escherichiacoli B, which polymerizes deoxyribonucleoside-5' diphosphates. Under appropriate conditions, the enzyme will add a single deoxyribonucleotide residue to a deoxyribo-oligonucleotide primer. At all stages, the enzyme activity copurified with the activity which will polymerize adenosine-5' diphosphate (polynucleotide phosphorylase...

The nucleotide sequence of a T4 tRNA with an anticodon for glycine has been determined using (32)P-labeled material from T4-infected cultures of Escherichiacoli. The sequence is: pGCGGAUAUCGUAUAAUGmGDAUUACCUCAGACUUCCAApsiCUGAUGAUGUGAGTpsiCGAUUCUCAUUAUCCGCUCCA-OH. The 74 nucleotide sequence can be arranged in the classic cloverleaf pattern for tRNAs. The anticodon of T4 tRNA(Gly) is UCC with a p...

a. calamus that grows in province of baluchistan and kerman in iran plays a major role in the revival of traditional medicine. despite the useful role of this plant in many ailments, its anti-bacterial properties are not well understood. in this study, we investigated anti-bacterial activities of rhizomes ware of a. calamus against various strains of bacteria such as staphylococcus aureus and e...

With the aim of engineering a strain of bacteria that could be used for bioremediation of cellulosic waste in radioactive environments, the gene for the secreted endoglucanase enzyme of Bacillus pumilis was decided to be cloned into the radiotolerant bacterium, Deinococcus radiodurans. The endoglucanase gene from B. pumilus was PCR amplified and cloned into Escherichiacoli DH5α using a pDrive v...