Following the discovery of ergosterol by Tanret (l), Gerard (2) observed that not only ergot, but fungi in general, contain it. Gerard was the first to recognize ergosterol in yeast, although Nageli (3) had earlier obtained a yeast “cholesterol” in crude form. In traces ergosterol is now known to be widely distributed in both plant and animal kingdoms, but the fungi, and particularly the yeasts...

1. A convenient synthesis of 24-methylene[23,25-(3)H(3)]dihydrolanosterol is described. 2. A general anaerobic-aerobic method for the incorporation of sterols into whole yeast cells is also described and illustrated by experiments with (3)H-labelled lanosterol. 3. The method was used to convert labelled 24-methylene-dihydrolanosterol into ergosterol, in good yield, by Saccharomyces cerevisiae. ...

This study shows that antifungal curcumin (CUR), significantly depletes ergosterol levels in Candida albicans. CUR while displaying synergy with fluconazole (FLC) lowers ergosterol. However, CUR alone at its synergistic concentration (lower than MIC50), could not affect ergosterol contents. For deeper insight of CUR effects on lipids, we performed high throughput mass spectroscopy (MS) based li...

We studied the relationship between amounts of fungi and ergosterol in soil to further evaluate the use of ergosterol as an indicator of soil fungal biomass. Soils from seven sites in central Iowa were analyzed for soil ergosterol content, total fungal hyphal length (living and non-living, using calcofluor as stain), and living fungal hyphal length (using fluorescein diacetate as stain) to: 1) ...

BACKGROUND Ergosterol has been considered the "fungal sterol" for almost 125 years; however, additional sterol data superimposed on a recent molecular phylogeny of kingdom Fungi reveals a different and more complex situation. METHODOLOGY/PRINCIPAL FINDINGS The interpretation of sterol distribution data in a modern phylogenetic context indicates that there is a clear trend from cholesterol and...

Eight antifungal agents were examined for effects on lipid biosynthesis and membrane integrity in Candida albicans. Lipids were labeled in vivo or in vitro with [14C]acetate and analyzed by thin-layer and gas chromatography. Membrane integrity was measured by a recently developed [14C]aminoisobutyric acid radiolabel release assay. The imidazole antifungal agents miconazole, econazole, clotrimaz...

Fungal Lipids, Ergosterol, Inhibition, Etaconazole, Fungicide The triazole fungicide etaconazole (C G A 64 251) interferes with the ergosterol biosynthesis in Ustilago maydis by inhibiting the C-14 demethylation o f the sterol nucleus. During the late log growth phase o f U. maydis a novel endogenous sterol metabolite (14x-methyl-ergosta-8,24(28)dien-3/?,6a-diol) was discovered and analyzed, wh...

Halotolerant and halophilic melanized fungi were recently described in hypersaline waters. A close study of the sterol composition of such fungi, namely Hortaea werneckii, Alternaria alternata, Cladosporium sphaerospermum, Cladosporium sp., and Aureobasidium pullulans revealed the dominance of ergosterol and the presence of 29 intermediates of its biosynthesis pathway. The presence or absence o...

Four mutants strains of Aspergillus fennelliae resistant to various polyene antibiotics have been analyzed for their sterol content. The mutant strains contained Delta(7,22,24(28))-ergostatrien-3beta-o1 and Delta(7,22)-ergostadien-3beta-o1 as the major sterols, whereas the wild types contained ergosterol, indicating that the mutants contain metabolic blocks for C(5)-C(6) dehydrogenation and C(2...

Sixty four samples of cocoa beans replicated in quadruplicates were collected from five warehouses from southwest Nigeria and examined for fungal loads, ergosterol and ochratoxin A The levels of all the variables obtained were further used as indices for cocoa grading into food quality, FoQ (erg < 5 mg/kg; OTA < 1 µg/kg), feed quality, FeQ (erg = 5~10 mg/kg; OTA in the range of 1.1~3.11 µg/kg),...