gelatin is a protein hydrocolloid. because of its unique characteristics such as gel forming, thickening, stabilizing, emulsifiing, foam and film forming, is widely used in food industries. in this research, in order to optimize the factors which affect enzymatic extraction method of gelatin ,the effects of three variables, enzyme concentrations(2-10 ppm), time(8-16 hours) and temperature(60-80...

objective: in recent decades, β-glucans have been used as important complementary and alternative medicines for numerous immunocompromised individuals and those with end stage of cancer terminal. the most active form of β-glucans is β(1,3)d-glucan and its  most common source is cell wall of candida albicans. recently it has been introduced as a nano particle design to be used as a carrier for d...

This study was done to evaluate various DNA and RNA extractions from archival FFPE tissues. A total of 30 FFPE blocks from the years of 2004 to 2006 were assessed with each modified and adapted method. Extraction protocols evaluated include the modified enzymatic extraction method (Method A), Chelex-100 extraction method (Method B), heat-induced retrieval in alkaline solution extraction method ...

abstract: aqueous enzymatic extraction of oil from pistacia khinjuk was performed using cellulase. the central composite design was used to optimize the parameters that are significant to the process. the influence of three regressors on the percentage of oil recovery from seed was evaluated using second-order polynomial multiple regression model. analysis of variance showed a high coefficient ...

ABSTRACT: Aqueous enzymatic extraction of oil from pistacia khinjuk was performed using cellulase. The central composite design was used to optimize the parameters that are significant to the process. The influence of three regressors on the percentage of oil recovery from seed was evaluated using second-order polynomial multiple regression model. Analysis of variance showed a high coefficient ...

Several common protein extraction protocols have been applied on seaweeds, but extraction yields have been limited. The aims of this study were to further develop and optimize existing extraction protocols and to examine the effect of enzymatic pre-treatment on bioaccessibility and extractability of seaweed proteins. Enzymatic pre-treatment of seaweed samples resulted in a three-fold increase i...

background: in patients with non-obstructive azoospermia (noa), vital spermatozoa from the tissue is obtained from testes by enzymatic treatment besides the mechanical treatment. objective: to increase the sperm recovery success of testicular sperm extraction (tese), with enzymatic digestion if no sperm is obtained from testis tissue by mechanical method. materials and methods: tissue samples w...

In this study, ultrasound- and enzyme-assisted extractions of albumin (water-soluble protein group) from defatted pumpkin (Cucurbita pepo) seed powder were compared. Both advanced extraction techniques strongly increased the albumin yield in comparison with conventional extraction. The extraction rate was two times faster in the ultrasonic extraction than in the enzymatic extraction. However, t...

Background New biorefinery concepts are necessary to drive industrial use of lignocellulose biomass components. Xylan recovery before enzymatic hydrolysis of the glucan component is a way to add value to the hemicellulose fraction, which can be used in papermaking, pharmaceutical, and food industries. Hemicellulose removal can also facilitate subsequent cellulolytic glucan hydrolysis. Results...

Biocatalytic processes often provide an ecological alternative to many chemical processes. However, further improvements in terms of the economic efficiency are required. In order achieve that, concept process integration is a promising option. Applying this within biocatalytic process, highly integrated apparatus working as reactive extraction centrifuge was developed and operated. For purpose...