Enteropathogenic Escherichia coli isolated from diarrheal children and adults were studied. Twenty-two OK groups were set up as enteropathogenic Eschericha to include : O26: K60, O28a, 28c : K73, O44: K74, O55: K59, O86: K61, O86: K62, O111: K58, O112a,112c : K66, 0119: K69, O124: K72, O125: K70, O126: K71, O127: K61, O86: K63, O128: K67, O136: K78, O143: Kx1, O144: Kx2, O146: K87, A-D (Alkales...

A total of 90 random samples of ready to-eat-pastry foods of meat pie, meat pizza and pasta pashamil. 30 samples of each were collected from different fast foods services at Kalyobia governorate to be examined bacteriologically for detection of pathogenic Escherichia coli microorganisms. The obtained results indicate that incidence of E.coli isolated from examined samples of pastry foods were 2...

to determine the contamination of traditional cheeses to e. coli o157:h7 this study was conducted in chaharmahal and bakhtiari province. two hundred cheeses samples were obtained randomly and bacteriological examinations were done on all isolates, by culture in selective enrichment media and after that, selective plating. suspected colonies were examined with o and h antisera for identification...

Isolates of the most common O serogroups of enteropathogenic Escherichia coli (EPEC) associated with infant diarrhea (designated class I) adhere to Hep-2 cells; the genes for this adhesin, termed EPEC adherence factor (EAF), are located on plasmids 50-70 MDa in size. Volunteers ingested 10(10) organisms of an O127:H6 Hep-2-adhesive class I strain (E2348/69) or its plasmid-minus, nonadhesive der...

To study the association of multidrug-resistant enteroaggregative Escherichia coli with persistent diarrhea in Kenyan children, stool specimens were obtained from 862 outpatients under 5 years of age from July 1991 to June 1993. E. coli O44 was identified as the sole bacterial pathogen in four patients experiencing at least 14 days of fever, vomiting, and diarrhea. Disk diffusion testing showed...

To compare the diarrheagenic Escherichia coli (DEC) identifications obtained between traditional O serotyping and modern virulence gene detection assays, we developed a multiplex real-time PCR assay by detecting six specific virulence genes for enteropathogenic E. coli (EPEC), enterohemorrhagic E. coli (EHEC), enterotoxigenic E. coli (ETEC), and enteroinvasive E. coli (EIEC). Among 261 clinical...

To quantitatively evaluate factors potentially affecting antibody coating of bacteria in urine, we developed an assay with enzyme-linked rather than fluorescein-conjugated immunoglobulin. Using the enzyme immunoassay (EIA) in an in vitro system in which concentrations of serotype O44 Escherichia coli and antibody titer to E. coli Orr O44 O antigen were known, we compared specimens run in parall...

The present study was conducted to detect the occurrence, serotype, genotype, phylogenetic relationship and antimicrobial resistance pattern of STEC from healthy goats of West Bengal, India. From the 125 faecal samples collected from healthy goats, 245 isolates were identified as Escherichia coli. The E. coli harbouring any gene for Shiga toxins (stx1/stx2) was detected in 36 (14.7%) of the 245...

the present study was conducted to detect the occurrence, serotype, genotype, phylogenetic relationship and antimicrobial resistance pattern of stec from healthy goats of west bengal, india. from the 125 faecal samples collected from healthy goats, 245 isolates were identified as escherichia coli. the e. coli harbouring any gene for shiga toxins (stx1/stx2) was detected in 36 (14.7%) of the 245...

acute infectious gastroenteritis is one of the most common infectious diseases of humans in the world. diarrhea is a significant cause of death in infants. it has been estimated that up to 15% of children in developing nations die of diarrhea before 3 years of age. this study has been carried out to investigate entheropathogenic bacteria from 558 specimens of children less than 5 years of age. ...