in this research, shape memory effect of polycaprolactone based polyurethane/poly(vinyl chloride) blends in which the pvc weight percentage are 10, 20, 30, 40, 60 and 80 were studied using dma apparatus. the compatibility, tension and dynamic-mechanical properties of these blends have been studied by the authors and this paper specifically deals with the tests results of shape memory effect. th...

We have discovered that addition of monomeric desAB fibrin to prothrombin leads to appearance of the thrombin-like activity of prothrombin towards S2238 chromogenic substrate. DesA and desABβ(15-42)2 fibrin forms did not cause any activation of prothrombin. From this observation we could suggested that amino acid residues of the 15-42 fragment of BβN-domain presented in desAB fibrin, cleaved in...

The aim of this work is to study the intermolecular interactions fibrin with D-domain-containing fragments fibrin(ogen): D-dimer and D-fragment. Materials methods. Human fibrinogen was obtained from human blood plasma by salt extraction using 16 % Na2SO4. content protein coagulated thrombin – 96-98%. Analytical size-exclusion chromatography for detection molecular complexes performed on Sepharo...

Using laser tweezers, we measured for the first time the forces of individual knob-into-hole interactions underlying fibrin polymerization. Exposure of A-knobs in desA-fibrin or its fragment from the central part of the molecule (N-terminal disulphide knot, NDSK) resulted in strong interactions with fibrinogen or fragment D (containing only a- and b-holes), producing a binding strength of appro...

Plasmin cleaves fibrin at or near sites involved in platelet recognition and may modulate platelet adhesion and spreading. Using an in vitro system, we have characterized the effects of limited plasmic degradation of polymerized fibrin on platelet adhesion and spreading. As shown by scanning electron microscopy, exposure to plasmin changed the tight fibrillar fibrin surface to a less dense stru...

A four-membered oxygen ring (oxetane) can be readily grafted into native peptides and proteins through site-selective bis-alkylation of cysteine residues present as disulfides under mild and biocompatible conditions. The selective installation of the oxetane graft enhances stability and activity, as demonstrated for a range of biologically relevant cyclic peptides, including somatostatin, prote...

Clostridium scindens American Type Culture Collection 35704 is capable of converting primary bile acids to toxic secondary bile acids, as well as converting glucocorticoids to androgens by side-chain cleavage. The molecular structure of the side-chain cleavage product of cortisol produced by C. scindens was determined to be 11β-hydroxyandrost-4-ene-3,17-dione (11β-OHA) by high-resolution mass s...

Fibrin forms the cohesive network of hemostatic plugs and thrombi, and it also provides the temporary matrix for initial support of healing and revascularization. Because cell proliferation is needed for revascularization after vessel injury, we have characterized structural requirements of fibrin needed to support cell proliferation on fibrin in vitro. Proliferation of cultured human endotheli...

•Chemical mutagenesis was pursued along the CDR3 loop of a single-domain antibody•Sites deemed accessible had diverse side chains screened for activity enhancement•Final mutant greatly enhanced and maintained other desired properties Great advances have been made over last four decades in therapeutic diagnostic applications antibodies. The maturation antibody candidates, however, remains signif...