Chicken antibodies have been reported to be an excellent alternative to the mammalian antibodies, especially if the antigen is from mammalians. Because chicken immunoglobulin Y (IgY) does not bind to protein G, in this study a indirect method for the purification of murine immunoglobulin G (IgG) subclass specific egg yolk antibodies is described. After preincubation of the chicken egg antibodie...

Egg-laying hens were immunized with the Echis carinatus venom and the resulting antibodies were extracted from egg yolk by four different purification methods. The chicken egg yolk antibodies were purified by the water dilution method, polyethylene glycol (PEG) and ammonium sulphate precipitation method, chloroform extraction and the Lithium sulphate precipitation method. These methods were com...

egg-laying hens were immunized with the echis carinatus venom and the resulting antibodies were extracted from egg yolk by four different purification methods. the chicken egg yolk antibodies were purified by the water dilution method, polyethylene glycol (peg) and ammonium sulphate precipitation method, chloroform extraction and the lithium sulphate precipitation method. these methods were com...

background : staphylococcus aureus strains exhibiting multiple antibiotic resistances are isolated from most communities and hospital infections. treatment of patients with these infections has been difficult. the aim of this study was to detect and extract, the egg yolk immunoglobulin y as a potential source of anti- s. aureus antibody. methods : specific igy was produced by immunizing hens wi...

recently it has been reported, that immunoglobulin y (igy) can be used instead of polyclonal antibodies extracted from mammals (igg) for the purpose of diagnosis and therapy. these antibodies are found to have better properties in terms of specificity and ease of large-scale production. in addition, igy binds neither to mammalian complement or fc-receptors nor does it interfere with rheumatoid ...

Aim: The aim was to study the seroconversion and development of egg yolk immunoglobulins in adult laying White Leghorn hens immunized against an isolate of enterotoxigenic Escherichia coli (ETEC) bearing K91 and K88ac antigens, obtained from diarrheic piglet. Materials and Methods: Adult laying White Leghorn hens were immunized with inactivated enterotoxic E. coli strain isolated originally fro...

The present study evaluated the potential use of immunoglobulin prepared from the egg yolk of hens immunized with Helicobacter pylori (immunoglobulin Y [IgY]-Hp) in the treatment of H. pylori infections. The purity of our purified IgY-Hp was 91.3%, with a yield of 9.4 mg of IgY per ml of egg yolk. The titer for IgY-Hp was 16 times higher than that for IgY in egg yolk from nonimmunized hens, and...

The outcome and protective efficacy of maternal antibodies elicited by DNA immunization to the large (L) hepadnavirus envelope protein were studied using the duck hepatitis B virus (DHBV) model. Following genetic immunization of breeding ducks with a DHBV L protein gene-bearing plasmid, specific and highly neutralizing antibodies were transferred from the sera of immunized ducks, via the egg yo...

The development of an assay for the quantification of chicken immunoglobulin (IgY) and data evaluation is being demonstrated. An enzyme immunoassay was established to perform the quality control of IgY purifications from the egg yolk and to control IgY producing cells in vitro. Each step of the test procedure and possible alternatives were described. The assay was characterized by special test ...