background: equine chorionic gonadotropin (ecg) is commonly used in association with a progestagen treatment to synchronize estrus of goats and ewes during breeding and non-breeding seasons. classical purification of the ecg from serum includes ph fractionation with metaphosphoric acid two ethanol precipitation steps as well as dialysis followed by fixed-bed chromatography. objectives: the aim ...

  Background: Equine chorionic gonadotropin (eCG) is commonly used in association with a progestagen treatment to synchronize estrus of goats and ewes during breeding and non-breeding seasons. Classical purification of the eCG from serum includes pH fractionation with metaphosphoric acid two ethanol precipitation steps as...

Background: Equine chorionic gonadotropin (eCG) is commonly used in association with a progestagen treatment to synchronize estrus of goats and ewes during breeding and non-breeding seasons. Classical purification of the eCG from serum includes pH fractionation with metaphosphoric acid two ethanol precipitation steps as well as dialysis followed by fixed-bed chromatography. Objectives: The aim ...

one which is now widely used for diagnostic purposes. The difficulties involved in making a permanent accurate record of the results, an essential part of the technique, are indicated by the variety of photographic methods that have been used, the majority of which are time-consuming and involve the use of special photographic equipment. We have been using a modification of a method first descr...

Cryoprecipitation was observed in defibrinated serum from Schistosoma japonicum-infected rabbits. Ouchterlony immunodiffusion, sodium dodecyl sulfate-polyacrylamide gels, and anti-cryoprecipitate antiserum demonstrated the presence of immunoglobulin G (IgG), IgM, C3, fibrinogen, and an alpha-macroglobulin. Parasite antigen was not detected. IgG and IgM from pooled cryoprecipitates failed to rea...

introduction: it has been reported that cardiovascular effects are among the possible health effects of being exposed to extremely low frequency electromagnetic fields (elf emss). material and methods: this study has been conducted to evaluate the long term effects of exposure to magnetic and electric fields on electrocardiogram signal changes. nine power transmission posts having different vol...

Antisera against whole cells of each Peptostreptococcus species (P. anaerobius, P. micros, P. parvulus, and P. productus) were produced in rabbits. When these antisera were reacted against sonically disrupted cells and culture supernatant fluids in Ouchterlony tests, lines of identity were obtained among the antigens from all the species and uninoculated culture medium. When the antisera were s...

Thirty-two antisera against the hepatitis-associated antigen (HAA) were studied for titer and specificity. Titers were expressed in milligrams of the antigen precipitated by one milliliter of the antiserum. Specificity was investigated by Ouchterlony double diffusion against 40 HAA-positive sera and a serum pool consisting of about 200 HAA-positive specimens. Antiserum titers ranged from 0.04 m...