the aim of this study was to investigate the association of bovine leukocyte antigen-drb3 alleles with peripheral blood mononuclear cell (pbmc) proliferation in response to staphylococcus aureus. the animals included in this study (n=347) were approximately of same age and comprised of f2 holstein-friesian ´ charolais (n = 155), holstein-friesian backcross (f0 holstein-friesian dams crossed wit...

Bovine leukocyte antigen (BoLA) DRB3 is a highly polymorphic gene in major histocompatibility complex(MHC) class II that plays a central role in immune responses and production factors. As of yet, molecular andevolutionary characteristics of BoLA-DRB3.2* have not been as fully understood as human and mouse.Therefore, we attempted to analyze variability and phylogeny of BoLA-DRB3.2* and illustra...

The bovine MHC (BoLA) class II DRB3 alleles are associated with polyclonal expansion of lymphocytes caused by bovine leukemia virus (BLV) infection in cattle. To examine whether the DRB3*0902 allele, one of the resistance-associated alleles, is associated with the proviral load, we measured BLV proviral load of BLV-infected cattle and clarified their DRB3 alleles. Fifty-seven animals with DRB3*...

potential relationships between amino acid motifs in the antigen binding groove of various alleles of the bovine major histocompatibility complex dr (bola-dr) molecule and peripheral blood mononuclear cell (pbmc) proliferation, in response to s. aureus and phytohemaglutinin, were investigated. the animals included in the study were approximately of the same age and comprised of pure holstein-fr...

The major histocompatibility complex (MHC) contains many genes that play key roles in initiating and regulating immune responses. This includes the polymorphic MHCI MHCII present epitopes to CD8+ CD4+ T-cells, respectively. Consequently, characterisation of repertoire MHC is an important component improving our understanding genetic variation determines outcomes In cattle, (BoLA) research has p...

The degree of genetic polymorphism at the DRB3 locus in the major histocompatibility complex (Mhc) of the North American bison was investigated by PCR and DNA sequence analysis. Nine different alleles were characterized in a selected sample of 20 animals. The genetic distances between alleles were as large as usually found at highly polymorphic Mhc loci in other species. A comparative analysis ...

Bovine leukemia virus (BLV) has spread worldwide and causes serious problems in the cattle industry owing to lack of effective treatments vaccines. BLV is transmitted via horizontal vertical infection, with high proviral load (PVL), which a useful index for estimating disease progression transmission risk, are considered major infectious sources within herds. PVL strongly correlates highly poly...

We sequenced exon 2 of the major histocompatibility complex (MHC) class II DRB3 gene from 471 individuals in four different Japanese populations of cattle (201 Japanese Black, 101 Holstein, 100 Japanese Shorthorn, and 69 Jersey cattle) using a new method for sequence-based typing (SBT). We identified the 34 previously reported alleles and four novel alleles. These alleles were 80.0-100.0% ident...