During last two decades, polysaccharides such as alginate (Alg) alone and in combination with other biopolymers are widely used in vaccine and drug delivery systems. The aim of the present work was to investigate the potential utility of microparticles made of alginate (Alg) as new vehicles for improving nasal vaccine delivery. For this purpose, diphtheria toxoid (DT) was chosen as a model an...

during last two decades, polysaccharides such as alginate (alg) alone and in combination with other biopolymers are widely used in vaccine and drug delivery systems. the aim of the present work was to investigate the potential utility of microparticles made of alginate (alg) as new vehicles for improving nasal vaccine delivery. for this purpose, diphtheria toxoid (dt) was chosen as a model anti...

background and objective: escherichia coli o157:h7, an emerging pathogen, causes severe enteritis and the extraintestinal complication of hemolytic-uremic syndrome. the goal of this study was to evaluate the conjugate of e. coli o157: h7 lipopolysaccharide (lps) with diphtheria toxoid (dt) as a candidate vaccine in mice model. material and methods: lps from e. coli o157:h7 was extracted by hot ...

Preparation of chitosan (CS) microspheres as a novel drug delivery vehicle for intranasal immunization using high, medium and low CS molecular weight (MW) was investigated in this study. Diphtheria toxoid (DT) was used as a model antigen. The emulsion-solidification method was adopted for microencapsulation of DT.  In the first step, following the purification of semi-crude DT by the ion-exchan...

Preparation of chitosan (CS) microspheres as a novel drug delivery vehicle for intranasal immunization using high, medium and low CS molecular weight (MW) was investigated in this study. Diphtheria toxoid (DT) was used as a model antigen. The emulsion-solidification method was adopted for microencapsulation of DT.  In the first step, following the purification of semi-crude DT by the ion-exchan...

 The aim of me present work was to investigate rhe potential utility of nanoparticles made of chitosan (CS) and also CS chemically modified with polyethylene glycol (CS-PEG) as new vehicles for improving na^al vaccine delivery. For mis purpose, diphtheria toxoid (DT) was chosen as a model antigen. DT was entrapped within nanoparticles made of CS of different molecular weight, and also made of C...

Nasal mucosal immunization is very attractive for vaccination to prevent various bacterial and viral infectious diseases because of induction of systemic and mucosal immune responses. The aim of the present study was to investigate the possibility of changing the immunization procedure of diphtheria toxoid (DT) from intramuscular or subcutaneous injection to intranasal administration. Intranasa...

preparation of chitosan (cs) microspheres as a novel drug delivery vehicle for intranasal immunization using high, medium and low cs molecular weight (mw) was investigated in this study. diphtheria toxoid (dt) was used as a model antigen. the emulsion-solidification method was adopted for microencapsulation of dt.  in the first step, following the purification of semi-crude dt by the ion-exchan...

On 1 January 1996, diphtheria-tetanus revaccination at the age of 5 years was implemented in the Danish Childhood Vaccination Programme. Initially, a combined DT vaccine containing 25 Lf diphtheria toxoid was used. Due to a high frequency of spontaneously reported adverse reactions, however, concerns were raised that the diphtheria dose was too high, and it was reduced to 6.25 Lf. This survey p...

the aim of me present work was to investigate rhe potential utility of nanoparticles made of chitosan (cs) and also cs chemically modified with polyethylene glycol (cs-peg) as new vehicles for improving na^al vaccine delivery. for mis purpose, diphtheria toxoid (dt) was chosen as a model antigen. dt was entrapped within nanoparticles made of cs of different molecular weight, and also made of cs...