نتایج جستجو برای: dependent rna polymerase (rdrp)

تعداد نتایج: 980160  

Journal: :iranian biomedical journal 0
فریبا اسماعیلی fariba esmaeili

background: rna interference (rnai) is a phenomenon uses double-stranded rna (dsrna) to specifically inhibit gene expression. the non-specific silencing caused by interferon response to dsrna in mammalian cells limits the potential of utilizing rnai to study gene function. duplexes of 21-nucleotide short interfering dsrna (sirna) inhibit gene expression by rnai. in some organisms, sirna can als...

Journal: :Journal of virology 1998
E Routhier J A Bruenn

At least eight conserved motifs are visible in the totivirus RNA-dependent RNA polymerase (RDRP). We have systematically altered each of these in the Saccharomyces cerevisiae double-stranded RNA virus ScVL1 by substituting the conserved motifs from a giardiavirus. The results help define the conserved regions of the RDRP involved in polymerase function and those essential for other reasons.

Journal: :Journal of virology 2004
Shuetsu Fukushi Shigeyuki Kojima Reiko Takai Fuminori B Hoshino Tomoichiro Oka Naokazu Takeda Kazuhiko Katayama Tsutomu Kageyama

Replication of positive-strand caliciviruses is mediated by a virus-encoded RNA-dependent RNA polymerase (RdRp). To study the replication of Norovirus (NV), a member of the family Caliciviridae, we used a recombinant baculovirus system to express an enzymatically active RdRp protein from the 3D region of the NV genome and defined conditions for optimum enzymatic activity. Using an RNA template ...

Journal: :Science 2004
S Gazzani T Lawrenson C Woodward D Headon R Sablowski

In RNA interference (RNAi), double-stranded RNA (dsRNA) triggers degradation of homologous messenger RNA. In many organisms, RNA-dependent RNA polymerase (RdRp) is required to initiate or amplify RNAi, but the substrate for dsRNA synthesis in vivo is not known. Here, we show that RdRp-dependent transgene silencing in Arabidopsis was caused by mutation of XRN4, which is a ribonuclease (RNase) im...

Journal: :The EMBO journal 2013
Stacey D Wagner Petro Yakovchuk Benjamin Gilman Steven L Ponicsan Linda F Drullinger Jennifer F Kugel James A Goodrich

RNA polymerase II (Pol II) is a well-characterized DNA-dependent RNA polymerase, which has also been reported to have RNA-dependent RNA polymerase (RdRP) activity. Natural cellular RNA substrates of mammalian Pol II, however, have not been identified and the cellular function of the Pol II RdRP activity is unknown. We found that Pol II can use a non-coding RNA, B2 RNA, as both a substrate and a...

2014
Kunduri Govind Arindam Bakshi Handanahal S. Savithri

Identification of viral encoded proteins that interact with RNA-dependent RNA polymerase (RdRp) is an important step towards unraveling the mechanism of replication. Sesbania mosaic virus (SeMV) RdRp was shown to interact strongly with p10 domain of polyprotein 2a and moderately with the protease domain. Mutational analysis suggested that the C-terminal disordered domain of RdRp is involved in ...

Journal: :Meta Gene 2021

Norovirus (NoV) is an etiological agent of acute gastroenteritis in humans. Genomic recombinations NoV occur mainly at the junction genes encoding RNA-dependent RNA polymerase (RdRp) and major capsid protein (viral 1, VP1), which may produce strains with novel combinations VP1- RdRp-genotypes. Here effect on changes proportions VP1-genotypes was examined through analysis observed frequencies Ja...

Journal: :Russian Journal of Physical Chemistry B 2021

A model of a multidomain complex is constructed using molecular modeling methods to explain the mechanism inhibitory effect favipiravir on RNA-dependent RNA polymerase (RdRp) SARS-CoV-2 coronavirus. As initial atomic coordinates, we use cryoelectron microscopy data for apo form RdRp virus and structure hepatitis C virus. After appropriate substitutions, an containing chains potential enzyme inh...

Journal: :BMB reports 2010
Meijuan Lin Shan Ye Yi Xiong Dawei Cai Jiamin Zhang Yuanyang Hu

Replication of positive-strand RNA virus is mediated by a virus- encoded RNA-dependent RNA polymerase (RdRp). To study the replication of Ectropis obliqua virus (EoV), a newly identified insect virus belonging to the family Iflaviradae, we expressed the RNA polymerase domain in Escherichia coli and purified it on a Ni-chelating HisTrap affinity column. It is demonstrated that EoV RdRp initiated...

Journal: :Cell 2015
Bo Liang Zongli Li Simon Jenni Amal A. Rahmeh Benjamin M. Morin Timothy Grant Nikolaus Grigorieff Stephen C. Harrison Sean P.J. Whelan

The large (L) proteins of non-segmented, negative-strand RNA viruses, a group that includes Ebola and rabies viruses, catalyze RNA-dependent RNA polymerization with viral ribonucleoprotein as template, a non-canonical sequence of capping and methylation reactions, and polyadenylation of viral messages. We have determined by electron cryomicroscopy the structure of the vesicular stomatitis virus...

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