نتایج جستجو برای: denaturing gradient gel electrophoresis (dgge)
تعداد نتایج: 242337 فیلتر نتایج به سال:
conclusions with regard to improving the understanding of the differences in the biodiversity of the fecal microbiota between rd infants and h infants, the findings of this study can provide a possible basis to reveal the relationship between rd and intestinal microbiota. objectives this study aimed to reveal the changes in the biodiversity of the fecal microbiota of infants with rd. patients a...
Denaturing gradient gel electrophoresis (DGGE) is believed to be the most powerful pre-screening method for mutation detection currently available, being used mostly on an exon-by-exon basis. Broad-range DGGE for the analysis of multiple fragments or an entire gene is rarely applied. We and others have already shown that one or two DGGE conditions are usually sufficient to analyse an entire gen...
We present a novel denaturing gradient gel electrophoresis (DGGE) method which characterizes multiclonal communities of Staphylococcus aureus. The spa PCR-based DGGE method simultaneously separates strains that differ in only one base, thereby revealing multiclonal colonization and infections.
بررسی تنوع جمعیتی سویه های یرسینیا روکری در ماهیان قزل آلای پرورشی استان آذربایجان غربی با روش dgge. yersinia ruckeri یکی از باکتری های خانواده انتروباکتریاسه می باشد که عامل اصلی بیماری دهان قرمز روده ای یا یرسینیوز می باشد. این باکتری گرم منفی به عنوان یکی از عوامل ایجاد خسارات سنگین اقتصادی وکاهش تولید در مزارع پرورشی ماهی قزل آلای رنگین کمان و آزاد ماهیان اقیانوس اطلس شناخته شده است که حدو...
Five years after the introduction of denaturing gradient gel electrophoresis(DGGE) and temperature gradient gel electrophoresis (TGGE) in environmental microbiology these techniques are now routinely used in many microbiological laboratories worldwide as molecular tools to compare the diversity of microbial communities and to monitor population dynamics. Recent advances in these techniques have...
A novel technique is reported for screening point mutations is genomic DNA: double gradient, denaturing gradient gel electrophoresis (DG-DGGE). Unlike conventional DGGE, which exploits a single gradient of denaturing chemicals (typically urea and formamide) along the migration path to force the two hetero- and two homo-duplexes to partially unwind and separate, DG-DGGE superimposes a second (po...
We applied double gradient-denaturing gradient gel electrophoresis (DG-DGGE) for the rapid detection of rifampin (RMP) resistance from rpoB PCR products of Mycobacterium tuberculosis isolates and clinical samples. The results of this method were fully concordant with those of DNA sequencing and susceptibility testing analyses. DG-DGGE is a valid alternative to the other methods of detecting mut...
A combination of denaturing gradient gel electrophoresis (DGGE) and a previously described multiplex PCR approach was employed to detect sourdough lactobacilli. Primers specific for certain groups of Lactobacillus spp. were used to amplify fragments, which were analyzed by DGGE. DGGE profiles obtained from Lactobacillus type strains acted as standards to analyze lactobacilli from four regional ...
The intestinal bacterial diversity of Stichopus japonicus was investigated using 16S ribosomal RNA gene (rDNA) clone library and Polymerase Chain Reaction/Denaturing Gradient Gel Electrophoresis (PCR-DGGE). The clone library yielded a total of 188 clones, and these were sequenced and classified into 106 operational taxonomic units (OTUs) with sequence similarity ranging from 88 to 100%. The cov...
نمودار تعداد نتایج جستجو در هر سال
با کلیک روی نمودار نتایج را به سال انتشار فیلتر کنید