Background: Polycyclic aromatic hydrocarbons (PAHs) are a predominant group of contaminants that have been shown to accumulate and persist in marine organisms. We evaluated the dose-dependent induction of the cytochrome P4501A (CYP1A) system in the Sturgeon fish, Huso huso after after exposure to PAHs, beta-naphthoflavone (BNF) and 3- methylcholantheren. Methods: The fish were treated by i.p...

Changes in ethoxyresorufin-O-deethylase (EROD) activity were monitored through an extended 6-month dietary exposure to determine the relationship between EROD activity and uptake of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) in brook trout, Salvelinus fontinalis. Brook trout were fed labeled TCDD during a 4-week loading phase and an 11-week maintenance phase to achieve whole-body concentrations...

We describe a statistically significant correlation between two well-characterized responses to dioxin-like compounds in birds; induction of 7-ethoxyresorufin-O-deethylase (EROD) activity in cultured hepatocytes, and embryo mortality. Data were obtained from a review of the literature. EROD EC50 values for 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and 6 polychlorinated biphenyls (PCBs) were st...

background: polycyclic aromatic hydrocarbons (pahs) are a predominant group of contaminants that have been shown to accumulate and persist in marine organisms. we evaluated the dose-dependent induction of the cytochrome p4501a (cyp1a) system in the sturgeon fish, huso huso after after exposure to pahs, beta-naphthoflavone (bnf) and 3- methylcholantheren. methods: the fish were treated by i.p. i...

Cytochrome P450 monooxygenase 1A (CYP1A) is induced by several planar toxic compounds, for example, polychlorinated biphenyls (PCBs) and the induction of this protein is often measured in terms of CYP1A-mediated 7-ethoxyresorufin-O-deethylase (EROD) activity. This study was aimed at developing this assay in the Cape hake species Merluccius capensis and Merluccius paradoxus (considered one stock...

Long-term programmes like the UK Clean Seas Environmental Monitoring Programme (CSEMP) rely on biological effects techniques, including biomarkers, to assess if chemical pollutants are affecting sentinel species in aquatic environment. In this study, we have applied mixed and fixed effect linear models long-term CSEMP dataset (2005-2018) evaluate factors such as region (location), sex, age, gon...

Measurement of ethoxyresorufin-O-deethylase (EROD) activity in fish is a well-established in vivo biomarker of exposure to certain planar halogenated and polycyclic aromatic hydrocarbons (PHHs and PAHs) and other structurally similar compounds (Bucheli and Fent 1995; Stegeman and Hahn 1994). EROD is a highly sensitive indicator of contaminant uptake in fish, providing evidence of receptor-media...

We evaluated chronic exposure of harlequin ducks (Histrionicus histrionicus) to hydrocarbons associated with the 2004 M/V Selendang Ayu oil spill at Unalaska Island, Alaska. We measured levels of hepatic 7-ethoxyresorufin-O-deethylase activity (EROD) in liver biopsy samples as an indicator of hydrocarbon exposure in three oiled bays and one reference bay in 2005, 2006, and 2008. Median EROD act...

Ethoxyresorufin-O-deethylase (EROD) and porphyrin induction responses of primary hepatocytes to halogenated aromatic hydrocarbons (HAHs) were examined in newly hatched domestic chickens, herring gulls, ring-billed gulls, double-crested cormorants, and Forster’s terns. Concentration–response relationships were determined for both biochemical responses in hepatocyte preparations derived from indi...

We measured concentrations of polychlorinated biphenyls (PCBs), polychlorinated dibenzo-p-dioxins (PCDDs), and polychlorinated dibenzofurans (PCDFs) in northern leopard frogs collected from the Green Bay ecosystem and explored the catalytic activity of hepatic cytochrome P450-associated monooxygenase (P450 enzyme) as a biomarker for exposure to aryl hydrocarbon receptor (AhR) agonists. The two ...