Calcium-activated protein for secretion (CAPS) is proposed to play an essential role in Ca2+-regulated dense-core vesicle exocytosis in vertebrate neuroendocrine cells. Here we report the cloning, mutation, and characterization of the Drosophila ortholog (dCAPS). Null dCAPS mutants display locomotory deficits and complete embryonic lethality. The mutant NMJ reveals a 50% loss in evoked glutamat...

This paper describes the architecture fo r the distributed CAPS system (DCAPS). The system accomplishes distributed software prototyping with legacy module reuse. Prototype System Description Language (PSDL), the prototyping language, is used to describe real-time software in the DCAPS system. PSDL specifies not only realtime constraints, but also the connection and interaction among software c...

This paper describes the DATA-CHASER Automated Planner/Scheduler (DCAPS) system which automated generation and repair of command sequences for the DATA-CHASER shuttle payload. DCAPS uses general Artificial Intelligence (AI) heuristic search techniques, including an iterative repair framework in which the system iteratively resolves conflicts with the state, resource, and temporal constraints of...

This paper describes the DATA-CHASER Automated Planner/Scheduler (DCAPS) system for automated generation and repair of command sequences for the DATA-CHASER shuttle payload. DCAPS uses general Artificial Intelligence (AI) heuristic search techniques, including an iterative repair framework in which the system iteratively resolves conflicts with the state, resource, and temporal constraints of t...

Molecular genetic research relies heavily on the ability to detect polymorphisms in DNA. Single nucleotide polymorphisms (SNPs) are the most frequent form of DNA variation in the genome. In combination with a PCR assay, the corresponding SNP can be analyzed as a derived cleaved amplified polymorphic sequence (dCAPS) marker. The dCAPS method exploits the well-known specificity of a restriction e...

Spines or trichomes on the fruit of cucumbers enhance their commercial value in China. In addition, glabrous mutants exhibit resistance to aphids and therefore their use by growers can reduce pesticide residues. Previous studies have reported two glabrous mutant plants containing the genes, csgl1 and csgl2. In the present study, a new glabrous mutant, NCG157, was identified showing a gene inter...

Chloroplast simple sequence repeats amplicons in 5 subspecies of Brassica oleracea were sequenced, and one chloroplast SNP was detected in amplicon ACP43. Through the introduction of an RsaI recognition site by adding one mismatch in the forward primer, combined with the increased primer length and raised annealing temperature, the dCAPS (derived cleaved amplified polymorphic sequences) marker ...

A dual mode current-programmed, current-output active pixel sensor (DCAPS) in amorphous silicon (a-Si:H) technology is introduced for digital X-ray imaging, and in particular, for hybrid fluoroscopic and radiographic imagers. Here, each pixel includes an extra capacitor that selectively is coupled to the pixel capacitance to realize the dual mode behavior. Pixel structure, operation and charact...

Genetic manipulation of organisms using CRISPR/Cas9 technology generally produces small insertions/deletions (indels) that can be difficult to detect. Here, we describe a technique to easily and rapidly identify such indels. Sequence-identified mutations that alter a restriction enzyme recognition site can be readily distinguished from wild-type alleles using a cleaved amplified polymorphic seq...