Abbreviations: AI: Artificial Insemination; BCF: Beat Cross Frequency; PI: Propidiumiodide; ZBA: Zona Binding Assay; DFI: DNA Fragmentation Index; HZA: Hemizona Assay; SCSA: Sperm Chromatin Structure Assay; AOT: Acridine Orange Test; SCD: Sperm Chromatin Dispersion Test; ROS: Reactive Oxygen Species; VSL: Straight Line Velocity; LIN: linearity; VCL: Curvilinear Velocity; CTC: Fluorescent Chlort...

abstract background: sperm parameters and motion kinetics are affected by cryopreservation. objective: the main purpose of the current study was to determine the effect of different concentrations of trolox as an antioxidant to freezing-thawing procedure on human sperm kinematic parameter. materials and methods: semen was collected from 20 normal donors and divided into five aliquots prior to c...

The effect of glucose in a modified Ham's F10 medium (MM) without hypoxanthine, phosphate and transition metals on human fertilization and sperm survival in vitro was determined. Mature human oocytes from in-vitro fertilization (IVF) patients or Percoll-washed human spermatozoa were randomly allocated to one of the treatment groups: normal Ham's F10, MM, MM with 5 mM glucose (HGMM) and MM with ...

The general population is exposed to phthalates through consumer products, diet, and medical devices. The present study explored whether phthalates, reproductive toxins in laboratory animals, were associated with altered sperm movement characteristics in men. Two-hundred twenty subjects provided a semen sample for computer-aided sperm analysis (CASA) and a urine sample for measurement of phthal...

The aim of this study was to determine the effects of co-Q10 and ellagic acid on sperm parameters after the freeze–thawing of Ghezel ram sperm. Twenty ejaculates from five Ghezel rams were collected in this study. Semen samples, which were diluted with a soybean lecithin (SL) based extender containing 0.25 mM ellagic acid, 0.5 μM co-Q10, 0.25 mM ellagic acid + 0.5 μM co-Q10 and no antioxidant (...

The present study investigated the existing relationship between computer assisted semen motility parameters and gametes motility duration, known to express semen quality and fertilizing capacity in fish. The objective was particularly to identify computer aided semen analysis (CASA) parameters that could be used as potential fertility predictors. Semen samples were collected from the beginning...

BACKGROUND Sperm parameters and motion kinetics are affected by cryopreservation. OBJECTIVE The main purpose of the current study was to determine the effect of different concentrations of Trolox as an antioxidant to freezing-thawing procedure on human sperm kinematic parameter. MATERIALS AND METHODS Semen was collected from 20 normal donors and divided into five aliquots prior to cryoprese...

The ability of three milk-based extenders to preserve the motility and viability cooled shipped semen produced by stallions after a long sexual rest was evaluated. In total, 21 ejaculates from 11 were collected 5–6 months inactivity diluted with Kenney, INRA 96, EquiPlus. Seven parameters evaluated – spermatozoa (VIT, %), total (MOT, progressive (PMOT, number per ml (prog M/ml, million/ml), ave...

BACKGROUND Voltage-dependent anion channel (VDAC), a channel protein, exists in the outer mitochondrial membrane of somatic cells and is involved in multiple physiological and pathophysiological processes. Up until now, little has been known about VDAC in male germ cells. In the present study, the relationship between VDAC and human sperm motility was explored. METHODS Highly motile human spe...