The effects of cooling, storage, glycerolization and spermatozoal numbers on fertility were investigated utilizing three stallions and 72 mares. Semen was extended in a cream-gel extender and inseminated: a) within 1 hr (fresh) at 38 C, b) after cooling for 2 hr to 5 C, c) after cooling for 2 hr plus the addition of 7% (v/v) glycerol, and d) after cooling for 24 hours. Onehalf of the mares were...

The aim of the present study was to evaluate the quality of raw and cooled semen in Icelandic stallions. Experiments were performed using seven stallions aged between 3 and 19 years. From each stallion, six ejaculates were collected, and semen quality was determined. Thereafter, the semen was split into eight equal parts and processed with and without centrifugation using the extenders INRA 82-...

Elevating the level of specific omega-3 fatty acids in equine semen by dietary modification can improve motion characteristics of cooled-stored and frozen-thawed sperm. Stallions, which produce semen that has poor tolerance to cooling and freezing, would benefit most from this dietary modification.

The aim of this study was to evaluate the effect of two different extenders (Skimmed Milk Glucose SMG or Lactose Egg Yolk LEY) on physical characteristics and fertility of fractionated donkey semen cooled at 5°C. For this, four Pêga donkeys were used as semen donors. The sperm rich fraction of the ejaculate was diluted preparing insemination doses containing 400 x 10 6 motile spermatozoa in a v...

The present study was aimed to test the efficacy of adding vitamins C or E to Tris-fructose-egg yolk diluent to increase Awassi ram sperm storage period at 5 ˚C. Semen samples from six mature Awassi rams were used in this study. The semen samples were diluted by Tris-glucose-egg yolk. Diluted semen sample was divided into three parts. The first part was added with 0.9 mg mL-1 vitamin...

This study aimed at assessing the sperm quality of the Amazon catfish, Leiarius marmoratus ¸ after refrigeration without extenders. After capturing the animals and stripping of semen, the following parameters were analyzed: progressive motility, motility quality score, duration of motility and sperm morphology. An aliquot of fresh semen from each male was kept at room temperature (28 ± 2°C) as ...

the present study was aimed to test the efficacy of adding vitamins c or e to tris-fructose-egg yolk diluent to increase awassi ram sperm storage period at 5 ˚c. semen samples from six mature awassi rams were used in this study. the semen samples were diluted by tris-glucose-egg yolk. diluted semen sample was divided into three parts. the first part was added with 0.9 mg ml-1 vitamin c, the sec...

The objective of this experiment was to evaluate spermatological parameters and fertilizing ability of short term stored semen in different extenders from endemic Abant trout (Salmo trutta abanticus T,1954). Semen was collected from 30 adult males by the hand stripping method without anesthesia. Having determined the main spermatological properties (volume, motility, movement duration, concentr...

The aims of this study were to assess and compare the effect of skim milk with (MEY) and without (SMI) egg yolk on canine spermatozoa incubated at 4oC in vitro and to evaluate the efficacy of MEY in vivo. Also, the effect of semen cooled storage before freezing was also evaluated in vitro. The ejaculates of 10 dogs were collected, pooled, centrifuged, and divided into 4 aliquots and diluted in ...

With the recent acceptance of frozen semen by the American Quarter Horse Association and the American Paint Horse Association, the overall interest and use of this breeding method has increased significantly in recent years. Proper handling and maintenance of semen frozen and stored in liquid nitrogen is critical to the success of artificial insemination with frozen semen. International distrib...