background: yeast infections are increasing cause of morbidity and mortality in immunocompromised patients. in order to perform a dna-based diagnostic test, availability of a rapid and easy-to-perform dna extraction protocol is essential. in the present study we evaluated colony-pcr as the easiest way to amplification of target dna. methods: instead of using templates of purified genomic dna, w...

introduction: streptomyces species are mycelial, aerobic gram-positive bacteria that are isolated from soil and produce a diverse range of antibiotics. streptomyces griseus produces the antibiotic, streptomycin and forms spores even in a liquid culture. the gene cluster for the production of streptomycin antibiotic contains strr gene that encodes strr, a pathway-specific regulator. then, this p...

The aim of this study was to determine the Nosema species by microscopic and molecular method its association with honeybee colony losses (Colony Collapse Disorder) in apiaries Urmia, Northwest Iran. For purpose, samples were collected from 840 colonies kept 120 five different location Urmia. specimens examined for presence spores. After DNA isolation, 16S rRNA gene evaluated using multiplex PC...

Introduction: Group B Streptococcus (GBS) is one of the most causes of neonatal infections. The bacterium colonizes genitourinary tracts of pregnant women and transmits to infants. The aim of this study was investigating colony PCR and culture methods to detection of GBS in pregnant women. Materials and methods: Hundred pregnant women, at the 35th and 37th weeks of pregnancy, were selected fro...

BACKGROUND Failure of colony PCRs in green microalga Chlorella vulgaris is typically attributed to the difficulty in disrupting its notoriously rigid cell walls for releasing the genetic materials and therefore the development of an effective colony PCR procedure in C. vulgaris presents a challenge. RESULTS Here we identified that colony PCR results were significantly affected by the accumula...