Introduction and Aim: Andrographolide is one of the main constituents Andrographis paniculata. An anti-collagenase property andrographolide yet to be explored. Thus, primary goal study assess andrographolides against standard bacterial collagenase. The secondary objective evaluate molecular docking between proteins periodontal pathogens [P. gingivalis (Arg Lys Gingipains), P. intermedia A. acti...

the aim of this study was to evaluate the effect of chlorhexidine (chx) application on immediate and two-month bond strength of a filled adhesive system. methods: forty eight non-carious human third molars without enamel of occlusal surface were randomly divided into two main groups according to chx application: control and chx. each main group was further subdivided into 2 subgroups depending ...

Etlingera elatior, Persicaria odorata, Centella asiatica, Senna alata and Phyllanthus emblica were reviewed for their in vitro anti-collagenase activity its total phenolic contents. Plant herbs play a vital role to promote the production of collagen which is component skin. Different solvents during plant extraction various parts used determine properties content. Generally, assay Folin-Ciocalt...

Background: Today, the use of maggot therapy has become widespread due to the increase in chronic ulcers in the world. The recombinant production of secreted enzymes from these larvae is a novel non-invasive method for the treatment of chronic ulcers. Lucilia sericata (L. sericata) collagenase (MMP-1) has been expressed in insect cells. Collagenase is an enzyme that is widely used in clinical t...

bacterial vaginosis (bv) has been considered to be the most prevalent infection found in sexually active women. bv is thought to play an important role in the premature rupture of membranes (prom) and preterm birth. preterm delivery accounts for a substantial percentage of low birth weight infants and perinatal mortality and morbidity. bacteroides and prevotella species have been isolated from ...

Real–time digestion of type I collagen molecules by bacterial collagenase from Clostridium histolyticum has been monitored using Dynamic Light Scattering method. Time dependencies for translation diffusion coefficient (Dt) and hydrodynamic radius (RH) on were obtained pure “collagen + collagenase” Tris–HCl buffer solution at different temperatures solutions with added CaCl2, ZnCl2 MgCl2 EDTA. I...

the aim of this study was to evaluate the effect of chlorhexidine (chx) application on immediate and two-month bond strength of a filled adhesive system. methods: forty eight non-carious human third molars without enamel of occlusal surface were randomly divided into two main groups according to chx application: control and chx. each main group was further subdivided into 2 subgroups depending ...

In this study, we aimed to develop a thrombus-targeting delivery system of collagenase bound to a monoclonal antibody, and to investigate the thrombolysis of an immune-conjugate in vitro and in vivo as well as the targeting effect. We prepared the immunizing conjugation of collagenase by the 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDCI) method. In order to conjugate collagenase and a mo...

Zoanthamine-type alkaloids display a wide spectrum of biological effects. This study aimed to examine the inhibitory effects of norzoanthamine and its ten homologues of zoanthamine class on human fibroblast collagenase by modeling a three-dimensional structure of the ligands at collagenase using energy minimization, docking, molecular dynamics simulation and MM-PB/GBSA binding free energy calcu...

The heterogeneity of human collagenases has been examined using a monoclonal antibody to neutrophil collagenase. This antibody inhibited collagenase activity and, when covalently coupled to Sepharose, bound both latent and active enzyme. Although human neutrophil collagenase was inhibited by the antibody, the activity of human skin and rheumatoid synovial collagenase was not significantly dimin...