A substantial amount of cytochrome oxidase subunit III (COIII) mRNA continues to be synthesized de novo in Trypanosoma brucei in the presence of actinomycin D, presumably by a DNA-independent transcription process. We describe the identification of negative-strand COIII RNA molecules, characterization of their termini, and the detection of RNA-dependent RNA polymerase activity. Three lines of e...

Partially edited mRNAs were selected by the polymerase chain reaction and sequenced. In the case of cytochrome b, 102 out of 106 clones displayed patterns of editing that were consistent with a strictly progressive 3' to 5' editing process, as predicted by the guide RNA model of RNA editing. In the case of cytochrome oxidase subunit III (COIII), 177 out of 304 clones displayed strictly progress...

The design of advanced metal–organic framework (MOF) catalysts for solar-driven conversion CO2 into syngas (CO/H2 mixture) is beneficial. Herein, the a joint MOF heterostructure consisting orderly assembled CoII- and CoIII-based Prussian blue analogs (PBAs) driven by their spontaneous lattice match in growth process reported. As-prepared H/CoIII[email protected]II-PBA cage mesocrystal exhibits ...

Photocatalytic Syngas Production In article number 2200085, Mingxiong Lin, Zanyong Zhuang, Yan Yu, and co-workers report the construction of an ordered CoIII[email protected]II-MOF heterostructure based on a spontaneous oriented attachment mechanism. CoIII-PBA exerts stronger affinity for CO2 molecules than CoII-PBA, thus serves as active sites, built-in electric field in protected]II-PBA heter...

Guide RNAs (gRNAs) for the editing of sites 1-8 of COIII mRNA and an "unexpected" partially edited COIII mRNA are encoded in the variable regions of specific kinetoplast DNA minicircles. The gRNAs can form 37 and 44 nucleotide perfect hybrids (allowing for G-U base pairs) with edited mRNAs. The gRNAs were detected on Northern blots and shown to have unique 5' ends situated close to the beginnin...

The carcinogenicity of estrogens in rodents and man has been attributed to either alkylation of cellular macromolecules and/or redox-cycling, generation of active radicals and DNA damage. Metabolic activation of estradiol leading to the formation of catechol estrogens is believed to be a prerequisite for its genotoxic effects. 4-Hydroxyestradiol is a potent inducer of tumors in hamsters. Previo...

Die ersten oktaedrisch koordinierten CoII-Komplexe mit redoxaktivem Bisguanidin-Liganden und acac-Coliganden wurden synthetisiert hinsichtlich ihrer Redoxchemie analysiert. N-H-Funktionen in einem partiell alkylierten Guanidino-Gruppen bilden N-H⋅⋅⋅O-Brücken zu den beeinflussen dadurch stark die Redoxchemie. Bei allen Komplexen ist erste 1 e−-Oxidation metallzentriert führt CoIII-Komplexen neut...

A series of CoIII carboxylate based upon N,N,O,O-tetradentate Schiff base ligand framework have been prepared. X-ray diffraction analysis confirms that these Schiff base CoIII carboxylate are all monomeric species with a six-coordinated central Co in their solid structures. The activities and polycarbonate selectivity of these complexes toward the copolymerization of epoxide (cyclohexene oxide ...

A cobalamin (Cbl) cofactor in corrinoid iron-sulfur protein (CoFeSP) is the primary methyl group donor and acceptor in biological carbon oxide conversion along the reductive acetyl-CoA pathway. Changes of the axial coordination of the cobalt ion within the corrin macrocycle upon redox transitions in aqua-, methyl-, and cyano-Cbl bound to CoFeSP or in solution were studied using X-ray absorption...

Context. There have been recent claims that a significant fraction of type 2 AGN accrete close or even above the Eddington limit. In type 2 AGN the bolometric luminosity (Lb) is generally inferred from the [OIII] emission line luminosity (LOIII). The key issue, in order to estimate the bolometric luminosity in these AGN, is therefore to know the bolometric correction to be applied to LOIII. A c...