abstract hop stunt viroid (hsvd) and citrus tristeza virus (ctv) are the causal agents of most important diseases of citrus. previously we reported a novel variant of hsvd that constantly associated with the yellow corky vein disease. here, 19 individual citrus leaves with yellow corky vein symptoms were sampled for ctv by elisa. in this study, most symptomatic samples were found to be infected...

Tristeza and Huanglongbing are the most important diseases of citrus caused by Citrus tristeza virus (CTV) and ‘Candidatus Liberibacter’ species, respectively. A reverse transcription duplex polymerase chain reaction (RT-d-PCR) was developed to detect these pathogens simultaneously from infected citrus plants. Consistence of the technique was validated and specificity of the primers was confirm...

Citrus tristeza virus (CTV, genus Closterovirus, family Closteroviridae) is the largest member of its taxonomic family. Viral particles, 2000 nm in length and 10–12 nm in diameter, are present in the phloem of infected plants. A single stranded positive sense RNA molecule approximately 20,000 nucleotides long constitutes its genome (BarBacterial expressed coat protein: development of a single a...

Abstract Hop stunt viroid (HSVd) and Citrus tristeza virus (CTV) are the causal agents of most important diseases of citrus. Previously we reported a novel variant of HSVd that constantly associated with the yellow corky vein disease. Here, 19 individual citrus leaves with yellow corky vein symptoms were sampled for CTV by ELISA. In this study, most symptomatic samples were found to be inf...

Citrus Tristeza disease, caused by CTV (Citrus tristeza virus), committs citrus plantations around the world and specifically attacks phloem tissues of the plant. The virus exists as a mixture of more or less severe variants, which may or may not cause symptoms of Tristeza. The objective of this study was to analyze the changes caused by CTV in the proteome of stems of sweet orange, as well as ...

Tristeza, caused by Citrus tristeza virus (CTV), has long been present in Florida but outbreaks of decline on sour orange rootstock were occasional events until the late 1970s. Sour orange rootstock was valued for the high quality of fruit produced and was widely used because of its tolerance of citrus blight, a disease of unknown etiology. Research was directed towards the selection and screen...

Background and Aims: Plant certification programs need reliable, fast, cheap and sensitive methods for detection of systemic pathogens with special interest in virus and viroid detection. Reverse transcriptase-polymerase chain reaction (RT-PCR) has been documented as an alternative assay for certification of plant propagating materials. The main object of the present study was the optimization ...

This document is one in a series designed to provide important information on the causal agent, symptoms, and transmission of exotic citrus diseases. can be used as an educational tool raise awareness about these diseases for scouting identification efforts. Disseminating industry may prevent their introduction spread Florida. will focus viral disease caused by isolates tristeza virus–stem pitt...