background: enterocin-p of enterococcus faecium p13 (entp) is of great interest as a food preservative and medicine due to its non-toxicity and broad antimicrobial spectrum in various ph, as well as its excellent thermal stability. however, recombinant production of entp is still in laboratory because of low productivity and complex purification process. objectives: in this study, we aimed to d...

The vacuolar chitinases of class I possess an N-terminal cysteinerich domain homologous to hevein and chitin-binding lectins such as wheat germ agglutinin and Urfica dioica lectin. To investigate the significance of this domain for the biochemical and functional characteristics of chitinase, chimeric genes encoding the basic chitinase A of tobacco (Nicotiana fabacum) with and without this domai...

Protochordate variable region-containing chitin-binding proteins (VCBPs) consist of immunoglobulin-type V domains and a chitin-binding domain (CBD). VCBP V domains facilitate phagocytosis of bacteria by granulocytic amoebocytes; the function of the CBD is not understood. Here we show that the gut mucosa of Ciona intestinalis contains an extensive matrix of chitin fibrils to which VCBPs bind ear...

Manduca sexta (tobacco hornworm) chitinase is a glycoprotein that consists of an N-terminal catalytic domain, a Ser/Thr-rich linker region, and a C-terminal chitin-binding domain. To delineate the properties of these domains, we have generated truncated forms of chitinase, which were expressed in insect cells using baculovirus vectors. Three additional recombinant proteins composed of the catal...

The Cel5 cellulase (formerly known as endoglucanase Z) from Erwinia chrysanthemi is a multidomain enzyme consisting of a catalytic domain, a linker region, and a cellulose binding domain (CBD). A three-dimensional structure of the CBD(Cel5) has previously been obtained by nuclear magnetic resonance. In order to define the role of individual residues in cellulose binding, site-directed mutagenes...

Evidence for the role of chitinases in biocontrol by Trichoderma species has been well documented.Chit42 lacks a chitin–binding domain (ChBD) which is involved in its binding activity to insoluble chitin. The objective of the present study was to enhance antifungal activity of T. harzianum by overexpression of wild type and hybrid forms of Chit42 containing chitin binding domain. To produce chi...

Chitin-binding proteins (CBPs) are present in many species and they act in a variety of biological processes. We analyzed a Pteromalus puparum venom apparatus proteome and transcriptome and identified a partial gene encoding a possible CBP. Here, we report cloning a full-length cDNA of a sequence encoding a chitin-binding-like protein (PpCBP) from P. puparum, a pupal endoparasitoid of Pieris ra...