background: stem cell therapy in small animal medicineis still in its infancy and few in vitro and in vivo research projectsregarding animal mesenchymal stem cells (mscs) have beencarried out. on the other hand, cell tracking is the first step of thecell-based therapies and is essential to recognize cell fate posttransplantation. objectives: the aim of this study was toisolate, characterize, an...

Objectives- To assess the ectopic bone formation in canine masseter muscle following the implantation of the natural bovine bone minerals (NBM) loaded with canine mesenchymal stem cells (MSCs).Design- Experimental study.Animals- four mongrel dogs.Procedures- Tripotent MSCs isolated from the canine bone marrow were loaded onto the NBM sponges and allowed to adhere. The cell-loaded scaffolds were...

Using a polyclonal antiserum against canine CD34, we previously found that CD34 is expressed on canine bone marrow progenitor cells in a manner analogous to that found in humans. To further characterize CD34+ cells and to facilitate preclinical canine stem cell transplant studies, monoclonal antibodies (MoAbs) were raised to CD34. A panel of 10 MoAbs was generated that reacted with recombinant ...

introduction: an investigation of bone, cartilage and fat differentiation potentials of canine bone marrow-derived mesenchymal stem cells. materials and methods: bone marrow aspirates were obtained from 10 canine’s iliac crest under general anesthesia. each sample was separately loaded on lymphodex and centrifuged at 1200 rpm for ten minutes to obtain mononuclear cell fraction. 5×104 cell/cm2 o...

  SUMMARY Up to 15 years ago, bone marrow transplantation was experimental, but in the recent years it has gained an important role in the field of Medicine, and it has clearly been established as a therapeutic modality for a wide range of diseases. During 1950-1955 when marrow transplant was first attempted in human, little was known about transplant antigens. Supportive care techniques such...

BACKGROUND The bone morphogenetic proteins (BMPs) belong to a unique group of proteins that includes the growth factor TGF-β. BMPs play important roles in cell differentiation, cell proliferation, and inhibition of cell growth. They also participate in the maturation of several cell types, depending on the microenvironment and interactions with other regulatory factors. Depending on their conce...

This study aimed to verify the body condition parameters that can be used characterise possible cases of neglect in dogs with visceral leishmaniasis. Fifty were study. The control group contained 11 dogs. Of 39 infected dogs, 25 and 14 included multisymptomatic oligo/asymptomatic groups, respectively. evaluated score, mass index, bone marrow fat percentage (Soxhlet method), supernatant content ...

Because of their unsurpassed potency in presenting antigens to naive T cells, dendritic cells are considered to be an important candidate in the development of immunotherapeutic strategies. Despite the high potential of dendritic cell-based immunotherapy, as a so-called dendritic cell vaccination, few clinical approaches using dendritic cell vaccination have been performed in the dog because of...

PURPOSE The aim of this study was to investigate the immunomodulatory effects of canine periodontal ligament stem cells on allogenic and xenogenic immune cells in vitro. METHODS Mixed cell cultures consisting of canine stem cells (periodontal ligament stem cells and bone marrow stem cells) and allogenic canine/xenogenic human peripheral blood mononuclear cells (PBMCs) were established followi...