Abbreviations: Ab, amyloid-b; AD, Alzheimer disease; APLP, amyloid precursor–like protein; APP, amyloid precursor protein; CaMKIIa, calciumcalmodulin kinase IIa; dox, doxycycline; FA, formic acid; GFAP, glial fibrillary acidic protein; mo/ huAPP695, mouse APP with a humanized Ab domain; PBS, phosphate-buffered saline; SDS, sodium dodecyl sulfate; swe/ind, Swedish/Indiana; tTA, tetracycline tran...

loss in mouse models of neurodegeneration. Behavior Genetics, 37, 79–100. Galton, F. (1869). Hereditary genius: An inquiry into its laws and consequences. London: Macmillan. Shors, T. J., & Matzel, L. D. (1997). Long-term potentiation: What’s learning got to do with it? The Behavioral and Brain Sciences, 20, 597–655. Silva, A. J., Stevens, C. F., Tonegawa, S., & Wang, Y. (1992). Deficient hippo...

A significant fraction of the total calciumcalmodulin-dependent protein kinase II (CaMKII) activity in neurons is associated with synaptic connections and is present in nerve terminals, thus suggesting a role for CaMKII in neurotransmitter release. To determine whether CaMKII regulates neurotransmitter release, we generated and analyzed knockout mice in which the dominant alpha-isoform of CaMKI...

ATP elicits Ca transients in cultured cerebellar granule neurons acting through specific ionotropic (P2X) and metabotropic (P2Y) purinergic receptors. In these neurons, application of L-Glutamate (L-Glu) immediately before ATP induced a prolonged reduction of ATP-mediated responses that remains at least 5 minutes after L-Glu wash out. alpha-amino-3-hydro-5-methyl-4-isoxazolpropionic acid (AMPA)...

Mitochondrial biogenesis is a critical adaptation to chronic energy deprivation, yet the signaling mechanisms responsible for this response are poorly understood. To examine the role of AMP-activated protein kinase (AMPK), an evolutionarily conserved fuel sensor, in mitochondrial biogenesis we studied transgenic mice expressing a dominant-negative mutant of AMPK in muscle (DN-AMPK). Both DN-AMP...

| Several signal molecules modulate the physiology of mammalian oocytes. Changes in the level of various signal molecules and their downstream impact on maturation promoting factor (MPF) during the achievement of meiotic competency remain ill understood. Therefore, the present study aimed to analyze levels of various signal molecules and MPF during the achievement of meiotic competency in rat o...

Functional Characterization of the Mammalian TRPV4 Channel: Yeast Screen Reveals Gain-of-Function Mutations Christina A. Doyle Transient receptor potential (TRP) channels are a class of six-transmembrane (6-TM) cation-permeable channels that mediate flux of calcium and sodium into cells, leading to depolarization as well as activation of calcium-mediated second-messenger signaling pathways. The...