objective(s): tadalafil can restore endothelial function and treat atherosclerosis. however, the effect of tadalafil on transplant arteriosclerosis remains unclear. in this study, we explore the effects of tadalafil on allograft vasculopathy. materials and methods: male brow-norway rats supplied aorta grafts for male lewis rats. all recipients were divided into 3 groups: saline as placebo (cont...

Diosgenin is a natural steroid sourced from plants and exhibits good analgesic anti-inflammatory activity. However, mechanism of action associated with the activity diosgenin not yet delineated. Hence, in present study, we explored on nitric oxide/cyclic guanosine monophosphate/adenosine monophosphate sensitive potassium channels (NO/cGMP/kATP). The role (NO/cGMP/kATP) Wistar rat was studied us...

Phosphodiesterase is an enzyme that degrades the phosphodiester bond in second messenger molecules cAMP and cGMP, regulates localization, duration, amplitude of cyclic nucleotide signaling within subcellular domains. Sildenafil a potent selective inhibitor type 5 cGMP-specific phosphodiesterase used clinically to treat erectile dysfunction pulmonary arterial hypertension. In this study, we exam...

Cell autophagy and apoptosis processes are of interest in drug development contribute to the chemotherapy outcomes patients receiving cancer treatment. The functional roles cyclic nucleotides cells include maintenance metabolic homeostasis. cGMP steroid compounds participate apoptotic autophagic events, modulate function multi-drug resistance proteins. Endogenous nucleotide ratios change with a...

Background: This study aimed to investigate Levisticum officinale hydroalcoholic extract (LOHE) effect on both cGMP signaling pathway and phosphodiesterase 5 (PDE5) gene expression pattern and to examine the role of LOHE in apoptosis induction of MCF-7 and MDA-MB-468 cell lines. Methods: The half maximal inhibitory concentration (IC50) of LOHE was examined in both cell lines using the MTT assay...

in this study, we aimed to investigate the peripheral antinociception effects of specificphosphodiesterase 5 (pde-5) inhibitor vardenafil on carrageenan-induced nociception in rats,and the role of calcium besides the l-arginine- nitric oxide (no)- cyclic guanosine monophophate(cgmp) pathway in these effects. hyperalgesia was induced by the intraplantar injection of 0.1ml fresh carrageenan solut...

background- cd24 is a cell adhesion molecule that has been implicated in metastatic tumor progression cells. our aim was clarify correlation between cd24 expression and camp:cgmp ratio in murine colorectal cancer cell line (ct26) after using cholera toxin. materials and methods- the ct26 cells were cultured in microtubes for assaying camp and cgmp; also the cells were cultured in flasks for ass...

background sildenafil (viagra) has been introduced to treat erectile dysfunction by acting as phosphodiesterase 5 inhibitor and hence accumulation of guanosine cyclic monophosphate (cgmp). on the other hands, the nitric oxide/cgmp signaling pathway has crucial role in synaptic plasticity processes like long-term potentiation (ltp) in the central nervous system considered as a model of learning ...