plackett-burman design was used for selection of important media components such as carbon and nitrogen sources and minerals which affect poly(hydroxybutyrate) production and cell growth of methylobacterium extorquens dsmz 1340. among the studied variables, nitrogen and phosphorus sources, mgso4 and most of the trace elements were found to be significant variables for phb production from methan...

An active strain, isolated from soil of Chhattisgarh, India, showed broad-spectrum antimicrobial activity against various pathogenic bacteria and fungi in glucose soybean meal broth. Strain was characterized as Streptomyces hygroscopicus MTCC 4003 based on 16S rRNA sequencing from Microbial Type culture Collection (MTCC), IMTECH, Chandigarh, India. Identification of the purified antimicrobial c...

Exopolysaccharides (EPS) of fungal origin have attracted special attention from researchers due to their multifarious applications in the food and pharmaceutical industries. In the present study, optimization of the process parameters for the production of exopolysaccharide by Schizophyllum commune AGMJ-1 was studied using one factor at a time (OFAT) method, Plackett-Burman design (PBD) and res...

background: a glycolipopeptide biosurfactant produced by pseudomonas aeruginosa strain ikw1 reduced the surface tension of fermentation broth from 71.31 to 24.62 dynes/cm at a critical micelle concentration of 20.80 mg/l. the compound proved suitable for applications in emulsion stabilization in food, as well as in cosmetic and pharmaceutical formulations. methods: in the present study, placket...

The research extended the work of Chomtee and Borkowski (2012) which compared response surface designs—central composite designs (CCDs), Box-Behnken designs (BBDs), small composite designs (SCDs), Plackett-Burman composite designs (PBDs) and uniform shell designs (USDs)—over a set of reduced models in a spherical design region for five design variables (k = 5) based on the three alphabetic opti...

Amylase (EC 3.2.1.1) enzyme has gained tremendous demand in various industries, including wastewater treatment, bioremediation and nano-biotechnology. This compels the availability of greater yields that can be achieved by employing potential amylase-producing cultures statistical optimization. The use Plackett–Burman design (PBD) evaluates medium components having two-level factorial designs h...

In the present study, Plackett–Burman design (PBD) followed by response surface methodology (RSM) was used to optimize the culture medium composition for laccase production from Pleurotus ostreatus IMI 395545. Influence of carbon, nitrogen (sources) and inducer were evaluated by Plackett–Burman design. The factors that induce positive effect on enzyme production were further optimized using cen...

Background: A glycolipopeptide biosurfactant produced by Pseudomonas aeruginosa strain IKW1 reduced the surface tension of fermentation broth from 71.31 to 24.62 dynes/cm at a critical micelle concentration of 20.80 mg/L. The compound proved suitable for applications in emulsion stabilization in food, as well as in cosmetic and pharmaceutical formulations. Methods: In the present study, Placket...

A solid state fermentation method was used to utilize wheat straw as substrates for citric acid production by using Aspergillus niger ATCC 9142. The Plackett Burman design (PBD) of experiments was used to test the relative importance of the variables affecting production such as moisture content, age of spore, inoculum size, initial pH of substrate, methanol conce...

Extracellular fungal cellulases are key enzymes for the degradation of lignocellulosic biomass. Greater production of these enzymes could reduce the cost of biofuels production. In this study, the basal medium for cellulase production by a Penicillium janthinellum mutant (EU2D-21) in submerged fermentation conditions was optimized using response surface methodology (RSM). Initial studies using ...