نتایج جستجو برای: bp fragment. in each reaction mixture
تعداد نتایج: 17088466 فیلتر نتایج به سال:
abstract in aggregate, active forms of reactive silica with mineral names are sometimes associated with sand and gravel in concrete mixture. alkali hydroxides originated from alkalis in the cement or other resources form an alkaline silica gel with this reactive silica which becomes swallowed and expanded during time causing damage to concrete. there have been growing researches on alkaline r...
abstract amino acids are building blocks of proteins, and play a vital role in living beings existence and their functionality. the interaction of these compounds with metal ions is of great importance to biochemists, and chemists, because their functions can be utilized as a model in understanding enzymes mechanism for transport of metal ions to tissues. among twenty essential amino acids w...
fire blight, a bacterial disease caused by erwinia amylovora (burr.) winslow et al. 1920 is considered as a serious disease on pome fruits. during recent years, this disease has caused serious damages to the fruit growing industry in iran. precise and reliable methods for identification and detection of the pathogen can help in its early control, prevent the disease spread and help eliminate th...
Continuous flow polymerase chain reaction (CFPCR) devices are compact reactors suitable for microfabrication and the rapid amplification of target DNAs. For a given reactor design, the amplification time can be reduced simply by increasing the flow velocity through the isothermal zones of the device; for flow velocities near the design value, the PCR cocktail reaches thermal equilibrium at each...
A polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) test was performed to investigate the allele frequencies of the insulin-like growth factor (IGF1 and IGF2 genes) in 90 broilerchickens. A 793 bp fragment of IGF1 gene and 1146 bp fragment of IGF2 gene were amplified and digested with HinfI and Hsp92II restriction enzymes,respectively. Two types of alleles of A and B...
a polymerase chain reaction-restriction fragment length polymorphism (pcr-rflp) test was performed to investigate the allele frequencies of the insulin-like growth factor (igf1 and igf2 genes) in 90 broilerchickens. a 793 bp fragment of igf1 gene and 1146 bp fragment of igf2 gene were amplified and digested with hinfi and hsp92ii restriction enzymes,respectively. two types of alleles of a and b...
Introduction: Dicrocoeliasis is a common disease of bile ducts and gallbladder of domestic and wild ruminants. This disease is caused by different species of dicrocoelium including Dicrocoelium dendriticum. The aim of this study was to identify pathological damages and molecular features associated with this parasite in ruminants. Materials and Methods: In this cross-sectional study, 180 fresh...
This study was carried out to identify polymorphisms of BMP15, GDF9 and BMPRIB genes in cross- bred of Romanov×shal sheep. For this reason 79 blood samples were collected from a flock in Gonbad kavous city in Golestan province. DNA was extracted using modified salting out method. Polymerase chain reaction was done by specific primers for amplification of two fragments with 141and 153 bp from ...
Invasive aspergillosis (1 is a life-threatening condition in immunocompromised patients. An early diagnosis is of great importance because early treatment may resolve this potentially fatal infection. Recently, the polymerase chain reaction (PCR) has been used successfully in detecting specific DNA of several pathogen. In this study, nested PCR was used to detect DNA specific for A!.pergiflus s...
An attempt has been made to analyze the distribution of the beta-lactoglobulin genotype in swamp buffaloes and Murrah buffaloes utilizing polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP). Blood samples were taken from 50 swamp buffaloes and 50 Murrah buffaloes. The DNA was extracted by the phenol-chloroform method. The PCR-RFLP was performed using beta-lactoglobulin ...
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