background: potyviruses are accounted for 40% of viral diseases of various crops including vegetables and legumes. potyviruses can be transmitted through plant sap, seeds and many aphid species. due to ease of spread of these viruses detection of such viruses are crucial as to the control of the incited diseases. objectives: this study compared the efficiencies of two couples of primers in thei...

Background: Potyviruses are accounted for 40% of viral diseases of various crops including vegetables and legumes. Potyviruses can be transmitted through plant sap, seeds and many aphid species. Due to ease of spread of these viruses detection of such viruses are crucial as to the control of the incited diseases. Objectives: This study compared the efficiencies of two couples of primers in the...

The occurrence of viral co-infection is a common phenomenon in cultivated and native plant species and can alter the dynamics of virus infection. In this study, disease progress was examined in single and mixed infections of Cucumber mosaic virus (CMV) and Bean yellow mosaic virus (BYMV) by measuring the rate of symptom development, disease severity and area under disease progress curve on infe...

We describe the design and production of a tobacco mosiac virus mutant that provides for specific and stoichiometric attachment of a wide variety of ligand-linker groups. As a result, specific ligands could be chemoselectively linked to the virion to produce highly diverse nanomolecular materials. These included semi-crystalline protein arrays and metallic ‘nanopipes’, as well as nanomolecular ...

The complete coding sequence for the trichodiene synthase gene from Fusarium sporotrichioides was introduced into tobacco (Nicotiana tabacum) under the regulation of the cauliflower mosiac virus 35S promoter. Expression of trichodiene synthase was demonstrated in the leaves of transformed plants. Leaf homogenates incubated with [(3)H]farnesyl pyrophosphate produced trichodiene as a major produc...

Snap bean (Phaseolus vulgaris L.) is a major crop in the Great Lakes region of the United States. The most significant production occurs in Wisconsin, Michigan, New York, Illinois, and Pennsylvania. Snap bean crops also are produced in the Pacific Northwest, Georgia, Florida, and Ontario, Canada. In 2005, 124,367 ha of snap bean were harvested with a farm gate value of approximately $400 millio...

Beginning in 2000, snap bean (Phaseolus vulgaris L.) crops exhibiting virus-like symptoms on the foliage (leaf distortion, yellow and green mottling, and mosaic) were increasing in frequency on a regional scale across the northern United States and Canada (10,20). These crops also produced fewer pods or pods that were either twisted or necrotic (hence unmarketable). Across the Midwest and north...