The simultaneous separation of bovine whey proteins [alpha-lactalbumin and beta-lactoglobulin (A+B)] and soybean proteins was performed, for the first time, by capillary electrophoresis. Different experimental conditions were tested. The most suitable consisted of 0.050 M phosphate buffer (pH 8) with 1 M urea and 1.2 mg/ml methylhydroxyethylcellulose, UV detection at 280 nm, 15 kV applied volta...

I3C-NMR studies of modified 6-lactoglobulins, cyanylated at the single thiol group, have provided evidence to support the location of the thiol at Cys-121 [ I J and useful information about the nuclear relaxation behaviour of the thiocyanate carbons of cyanylated thiol groups in proteins [2]. The O-lactoglobulin monomer contains two tryptophan residues. Trp-19 is buried deep inside the hydropho...

The study was conducted to find the association of -Lactoglobulin locus with the milk performance traits in 157 lactating Sahiwal cows maintained at Cattle yard, NDRI, Karnal. Two different variants of -Lactoglobulin were detected from DNA by PCR-RFLP. It was found that the frequency of B allele was predominantly high (0.84± 0.03) than that of A allele (0.16± 0.03) in the population studied. ...

Camel (Camelus dromedarius) whey proteins were detected and compared to bovine whey proteins using size exclusion chromatography columns on HPLC. Camel whey proteins such as serum albumin and a-lactalbumin appear to possess molecular weights similar to the respective bovine whey proteins. Camel whey lacks b-lactoglobulin and consists of large amount of serum albumin, compared to bovine whey. Ca...

b-lactoglobulin (coded by the b-lg gene) is the major milk whey protein in ruminants. studies have shown that this protein is polymorphic in many breeds of sheep as a result of a single base pair substitution in the b-lg gene that also gives rise to rsai restriction fragment length polymorphism (rflp). blood samples were collected from 391 animals belonging to 5 iranian and 6 russian sheep bree...

Genetic polymorphism at the β-lactoglobulin (β-LG) loci in indigenous sheep breeds (Tsigai, Racka, Pramenka) was determined. Altogether 904 sheep were genotyped for the presence of the A, B and C alleles of β-lactoglobulin by PCR-RFLP. The AB genotype was the most common and the β-lactoglobulin A was the most frequent in the Cokanski Tsigai (54%), while the B allele was the most common in the R...

as 'goat lactoglobulin' gave a single peak on electrophoresis at pH 8.0 in barbiturate buffer, but three boundaries separated at pH 7-0 and at least two at pH 4-2-6-0 after 2 hr. at 4-5 v/cm., I= 01. The two main components had their isoelectric points at pH 5-2 and 5-9 respectively in sodium acetate buffer, I= 0-1. 'Goat lactoglobulin' thus behaved in much the same way as crystalline bovine ,B...

Biopolymer nanoparticles can be formed by heating globular protein/polysaccharide mixtures above the thermal denaturation temperature of the protein under pH conditions where the two biopolymers are weakly electrically attracted to each other. In this study, the influence of polysaccharide linear charge density on the formation and properties of these biopolymer nanoparticles was examined. Mixe...

The interactions of β-lactoglobulin AB in the presence of cationic surfactants such as Cetyltrimethylammonium bromide and Cetyltrimethylammonium p-Toluenesulfonate have been investigated using a variety of experimental techniques such as conductivity, UV-Vis spectrophotometry and fluorimetry. The conductivity of surfactants aqueous solutions with β-lactoglobulin shows that the cmc of cationic s...