fifty six holstein lactating cows (71 days in milk; 35 kg/day milk production) were employed in a complete block randomized design of repeated measurements. cows were randomly allocated to two treatments based on their parity. cows in group 1 were fed a balanced total mixed ration (600 g concentrate /kg dm and 400 g forages /kg dm) with 50 g rumen protected methionine (rpm) per head per day (me...

Although exerting valuable functions in living organisms, nonesterified fatty acids (NEFAs) can be toxic to cells. Increased blood concentration of oleic acid (OLA) and other fatty acids is detected in many pathological conditions. In sepsis and leptospirosis, high plasma levels of NEFA and low albumin concentrations are correlated to the disease severity. Surprisingly, 24 h after intravenous o...

Most cows encounter a state of negative energy balance during the periparturient period, which may lead to metabolic disorders and impaired fertility. The aim of this study was to assess the potential of milk fatty acids as diagnostic tools of detrimental levels of blood plasma nonesterified fatty acids (NEFA), defined as NEFA concentrations beyond 0.6 mmol/L, in a data set of 92 early lactatin...

Minerals and some biochemical parameters determined from blood serum analyses in left displacement abomasum (LDA) affected and healthy dairy cows to evaluate the effect of displacement abomasum on mineral status, energy metabolism and liver function. Samples were collected from 60 affected cows and 60 healthy control cows matched with cases, based on herd, parity, and stage of lactation. Concen...

OBJECTIVE Gluteo-femoral, in contrast to abdominal, fat accumulation appears protective against diabetes and cardiovascular disease. Our objective was to test the hypothesis that this reflects differences in the ability of the two depots to sequester fatty acids, with gluteo-femoral fat acting as a longer-term "sink." RESEARCH DESIGN AND METHODS A total of 12 healthy volunteers were studied a...

Fatty liver is a metabolic disorder of dairy cows in early lactation, and approximately half of multi-parous dairy cows experience a moderate to severe fatty liver at calving. Since the occurrence of fatty liver in buffalo is not known, the aim of this study was to evaluate the hepatic content of triacylglycerols (TAG) and total lipids (TL), and the serum content of non-esterified fatty acids (...

Measurement of concentrations glucose and nonesterified fatty acids (NEFA) in blood is common nutrition physiology studies. Proper collection preparation conditions the have been less well studied dairy cattle. The objective this experiment was to determine NEFA prepared with different anticoagulants (heparin vs. EDTA), use fluoride as a glycolysis inhibitor, time until centrifugation (<30 min ...