background: the american foulbrood disease (afb), caused by paenibacillus larvae subsp. larvae, is one of the main plagues affecting honey bee (apis mellifera l.) colonies, and it has a high negative impact on beekeepers worldwide. objectives: the aim of this research was to describe the evidence of paenibacillus larvae larvae contamination of honey bee colonies, in the east azerbaijan province...

american foulbrood (afb) is the most serious brood disease of the honey bee. traditional methods are reliable but rather slow simply because they are based on biochemical, morphological and physiological identification of cultivated isolates. the aim of this study is the detection of paenibacillus larvae larvae spores in honey and diseased larvae samples by culture and pcr. therefore 54 samp...

American foulbrood (AFB) is the most serious brood disease of the honey bee. Traditional methods are reliable but rather slow simply because they are based on biochemical, morphological and physiological identification of cultivated isolates. The aim of this study is the detection of Paenibacillus larvae larvae spores in honey and diseased larvae samples by culture and PCR. Therefore 54 samp...

American foulbrood (AFB) disease is caused by the sporeforming bacterium Paenibacillus larvae larvae. Traditional diagnosis is based on culture technique is time and laboratory work consuming. In this study with standard strain, PCR was developed by specific primers and PCR products were electrophoresed on 0.8 % agarose gel. The PCR primers were selected on the basis of the 16S rRNA gene and am...

american foulbrood (afb) disease is caused by the sporeforming bacterium paenibacillus larvae larvae. traditional diagnosis is based on culture technique is time and laboratory work consuming. in this study with standard strain, pcr was developed by specific primers and pcr products were electrophoresed on 0.8 % agarose gel. the pcr primers were selected on the basis of the 16s rrna gene and am...

After more than a century of American Foulbrood (AFB) research, this fatal brood infection is still among the most deleterious bee diseases. Its etiological agent is the Gram-positive, spore-forming bacterium Paenibacillus larvae. Huge progress has been made, especially in the last 20 years, in the understanding of the disease and of the underlying host-pathogen interactions. This review will p...

The bacterial pathogen Paenibacillus larvae subsp. larvae (P. l. larvae), is the etiological agent of American foulbrood, an extremely contagious and disastrous disease of honeybee brood. In case of American foulbrood the destruction of infected colonies is often considered the only workable control measure. Therefore, the ability to diagnose this disease properly is important to prevent unnece...

Introduction American Foulbrood (AFB) is one of the most threatening epidemic brood diseases in honey bee colonies. This disease is caused by the pathogen Paenibacillus larvae larvae. According to the common animal health law in Germany the outbreak of AFB has to be reported to veterinarian service. The veterinarian has to decide about eradication of bee colonies, cleaning and disinfection as w...

Background: The American foulbrood disease (AFB), caused by Paenibacillus larvae subsp. larvae, is one of the main plagues affecting honey bee (Apis mellifera L.) colonies, and it has a high negative impact on beekeepers worldwide. OBJECTIVES: The aim of this research was to describe the evidence of Paenibacillus larvae larvae contamination of honey bee colonies, in the East Azerbaijan Province...

American foulbrood (AFB) is one of the most destructive diseases of the honey bee. This infectious disease causes a great economic loss in beekeeping industry worldwide. This severe brood infection is caused by the spore forming bacterium, Paenibacillus larvae subspecies larvae White (formerly: Bacillus larvae subsp. larvae). AFB was not detected in Egypt till about two years, however the disea...