background: tuberculosis (tb) remains an important health problem throughout the world. despite its significance in public health, mechanisms of protective immunity against mycobacyerium tuberculosis in humans have not yet been understood. objective: to evaluate cell mediated immune response against purified ag 85, ppd and phytohemagglutinin (pha) in patients with tuberculosis and healthy tuber...

Antigen 85 (Ag 85) complex, a component of M.tuberculosis bacilli (MTB) have been the focus of extensive research for diagnosis of tuberculosis for last several years. The aim of the present study to evaluate the status of primary tuberculosis infection in confirmed tuberculous meningitis (TBM) patients by estimating Ag 85 complex in Cerebrospinal Fluid (CSF) and serum specimen. A prospective s...

BACKGROUND Diagnosis of tuberculosis (TB) remains problematic despite many new advanced diagnostic methods. A reliable and rapid diagnostic test, which could be performed in any standard pathology laboratory, would help to obtain definitive early diagnoses of TB. In the present study we describe a prospective evaluation for demonstrating Antigen (Ag) 85 complex in the sera from TB patients. M...

PURPOSE In the present study booster efficacies of Ag85 B, Bacillus Calmette-Guerin (BCG), and Ag85B peptides were evaluated using prime boost regimes in BALB/c mice. MATERIALS AND METHODS Mice were primed with BCG vaccine and subsequently boosted with Ag85B, BCG and cocktail of Ag85B peptides. RESULTS Based on analysis of immune response it was observed mice boosted with Ag85B peptides sho...

Protective immunity against Mycobacterium tuberculosis requires CD4+ lymphocyte-mediated immune responses and IFN-gamma activity. As the primary portal of entry of M. tuberculosis is the lung, pulmonary immune responses against multiple M. tuberculosis Ags were compared between both M. tuberculosis-exposed tuberculin skin test-positive healthy household contacts (HHC) of patients with active sp...

Background: Diagnosis of tuberculosis (TB) remains problematic despite many new advanced diagnostic methods. A reliable and rapid diagnostic test, which could be performed in any standard pathology laboratory, would help to obtain definitive early diagnoses of TB. In the present study we describe a prospective evaluation for demonstrating Antigen (Ag) 85 complex in the sera from TB patients. Me...

The concept of school sport on which we are basing our experience is set in the Historical Territory of Bizkaia (Basque Country, Spain) and this is regulated by Bizkaia’s Diputación Foral (Provincial Council), governed by the regional regulations of the Basque Government. This government takes the view that School Sport covers sport activities at teaching centres outside actual school teaching ...

introduction: tuberculosis (tb) caused by mycobacterium tuberculosis remains a major worldwide health problem. the only tb vaccine currently available is anattenuated strain of mycobacterium bovis termed bacillus calmette -guerin (bcg).the efficacy of bcg remains controversial. mycobacterium secretory proteins are generally considered important antigens for protection against tb. a major protei...

Background: Tuberculosis (TB) remains an important health problem throughout the world. Despite its significance in public health, mechanisms of protective immunity against Mycobacyerium Tuberculosis in humans have not yet been understood. Objective: To evaluate cell mediated immune response against purified Ag 85, PPD and Phytohemagglutinin (PHA) in patients with tuberculosis and healthy tuber...

A media consisting of isatin-Schiff bases and its inclusion complexes with β-cyclodextrine was developed to maximize the production of antibiotics Hexaene H-85 and azalomycine B by Streptomyces hygroscopicus CH-7. The media with β-cyclodextrine inclusion complex of isatin-3-thiosemicarbazone resulted in the maximum antibiotics concentration of 493 μg/cm for Hexaene H-85 and 191 μg/cm for azalom...