1. The effects of synthetic fluorocitrate were studied on: (a) the oxidation of citrate and cis-aconitate by rat liver mitochondria; (b) the activity of the aconitate hydratase found in the liver cell sap; (c) the activity of the aconitate hydratase solubilized from liver mitochondria. 2. Fluorocitrate was found to be a potent inhibitor of oxidation of citrate but only a weak inhibitor of oxida...

1. The effect of biologically synthesized and purified fluorocitrate on the metabolism of tricarboxylate anions by isolated rat liver mitochondria was investigated, in relation to the claim by Eanes et al. (1972) that this fluoro compound inhibits the tricarboxylate carrier at concentrations at which it has little effect on the aconitate hydratase activity. 2. That the inhibitory action of fluo...

Fluoroacetate is a potent poison of animals. It is metabolized to fluorocitrate, which is a powerful inhibitor of mammalian aconitate hydratase [aconitase; citrate (isocitrate) hydrolyase] (Peters, 1952). Fluoroacetate and fluoroacetamide, as systemic insecticides for plants, are taken up by the roots or absorbed by the leaves and are then translocated to other parts of the plant. The plants sh...

background: in this study, the toxicity of the different xenobiotics was tested on the fruit fly drosophila melanogaster model system.  methods: fly larvae were raised on food supplemented with xenobioticsat different concentrations (sodium nitroprusside (0.1-1.5 mm), s-nitrosoglutathione (0.5-4 mm), and potassium ferrocyanide (1 mm)). emergence of flies, food intake by larvae, and pupation hei...

Cultured skin fibroblasts from a 3 yr old girl with severe, diffuse neurologic disease and persistant lactic acidosis, oxidized radioactive citrate, palmitate, and pyruvate at less than one-third the rate of control cells. Her fibroblasts oxidized isocitrate and glutamate at rates comparable with controls. In disrupted cells from this patient, the activity of aconitate hydratase appeared normal...

The Escherichia coli and recombinant human cytosolic aconitases are inactivated by O2-., with a rate constant of approximately 3 x 10(7) M-1 s-1; the corresponding value for the porcine mitochondrial aconitase is approximately 0.8 x 10(7) M-1 s-1. Nitric oxide, which is reported to inactivate aconitase, did not do so at a perceptible rate, while incubation with peroxynitrite led to a rapid loss...