Phosphotriesterase, isolated from the soil-dwelling bacterium Pseudomonas diminuta, catalyzes the detoxification of organophosphate-based insecticides and chemical warfare agents. The enzyme has attracted significant research attention in light of its possible employment as a bioremediation tool. As naturally isolated, the enzyme is dimeric. Each subunit contains a binuclear zinc center that is...

Binary and ternary separations of heavy metals, e.g. Zn–Bi, Mn–Pb, Cd–Pb, Mn–Bi–Mo, and Cr–Pb–Bi on columns of titanium(IV) tungstosilicate and Zn–Ni, Mo–Pb, Hg–Pb, Zn–Cr–Bi, and Zn–Ni–Bi on titanium(IV) tungstophosphate, have been demonstrated. The adsorption and elution of important metal ions such as Mo, Al, and As on titanium(IV) tungstosilicate and Hg, Fe, and Cr on titanium(IV) tungstopho...

The extraction of Cu(II), Ni(II), Co(II), Cd(II), Zn(II), Pb(II) and Mn(II) with potassium-dihydro-bispyrazolyl-borate (H(2)BPz(2)(-)) in dichloromethane has been studied. Extraction constants (logK(ex)) have been calculated for all metal systems and were compared with those obtained with dibenzoylmethane (DBM), thenoyltrifluoroacetone (HTTA) and 1-phenyl-3-methyl-4-benzoyl-5-pyrazolone (HPMBP)...

The geochemical behavior of zinc, lead and copper from sulfidic tailings in a mine site with potential to generate acidic drainage (pyrite (55%) and sphalerite (2%)) is reported in this paper. The mining area is divided in two zones, considering the topographic location of sampling points with respect to the tailings pile: (a) outer zone, out of the probable influence of acid mine drainage (AMD...

The activation of DNase I by Mg, Mn, Co, Ni, Fe, Cd, Zn, Ba, Sr, Ca, and Cu ions has been studied by several methods, at different pH and salt concentrations. Mg, Mn, and Co are the best activators for initial stages of degradation. A synergistic effect is shown only by the pair Mg-Ca. Optimal pH of action is always situated at 6.5. DNase II is activated to about the same degree by alkaline ear...

Trace metal ions such as Zn(2+), Fe(2+), Cu(2+), Mn(2+), and Co(2+) are required cofactors for many essential cellular enzymes, yet little is known about the mechanisms through which they enter into cells. We have shown previously that the widely expressed ion channel TRPM7 (LTRPC7, ChaK1, TRP-PLIK) functions as a Ca(2+)- and Mg(2+)-permeable cation channel, whose activity is regulated by intra...

BACKGROUND Metal pollution of waterways directly affects human health and can impact the food chain. Seafood living in polluted water can accumulate trace metals. The purpose of this study was to analyze the toxic metals Pb and Cd and the dietary essential metals Zn and Mn in 120 cans of tuna species from four different brands processed in Iran and purchased in 2012. RESULTS The mean level of...

Profiles of some trace elements (Fe Mn, Zn, Cu, pb and Cd) were detected in saline water and surficial sediment of Wadi El-Natrun depression lakes. The trace elements concentrations largely indicate the influence of weathering of terrigenous sources on land. The order of the studied trace elements in brine lakes of Wadi El-Natrun from high to low concentrations are Mn > Pb > Fe > Zn > Cu > Cd a...

the reaction of an equivalent of 2- mercaptoethyl ammonium chloride with an equivalent of 2,6-diformyl -4- methyl phenol produced monoamine which was reacted with half equivalent of 1,2-diamine ethane to produce ethylene-bis-6-(2-mercapto-ethyl-imine)-methyl -4- methyl phenol [h4l]. the ligand was reacted with metal ions[cr(iii), mn(ii), fe(ii), co(ii), ni(ii), cu(ii), zn(ii), cd(ii), and hg(ii...

Replacement of a non-bridging oxygen in the phosphate diester bond by a sulfur has become quite popular in nucleic acid research and is often used as a probe, for example, in ribozymes, where the normally essential Mg(2+) is partly replaced by a thiophilic metal ion to reactivate the system. Despite these widely applied rescue experiments no detailed studies exist quantifying the affinity of me...