microscopy as “field based test” was compared with is900 blood pcr for the diagnosis and estimationof prevalence of mycobacterium avium subspecies paratuberculosis (map) in domestic livestock withclinical johne’s disease (jd). of 252 animals screened, 39.3 and 13.1% were positive by faecal microscopyand blood pcr, respectively. proportional agreement between “microscopy” and blood pcr was subst...

AIMS To evaluate a safe sputum processing method for detection of tuberculosis in developing countries. METHODS A sample processing method was developed in which acid fast bacilli were killed with 1% sodium hypochlorite and concentrated by flotation on a layer of xylene before staining by the Ziehl Neelsen or auramine O methods. RESULTS Best results were obtained by auramine O staining afte...

background: tuberculosis continues to be a serious public health problem causing nearly three million deaths per year all over the world. despite major improvement in diagnosis, it is not possible to control the disease in the absence of surveillance treatment, and follow-up programs. objectives: this research was designed to study the frequency of mycobacterium tuberculosis among specimens ref...

Background: Lymph node tuberculosis (LNTB) is the leading form of extra pulmonary tuberculosis and common in our country. Acid Fast Bacilli (AFB) detection by Ziehl-Neelsen (ZN) method in the fine needle aspirate from these lymph nodes is confirmatory. Objective: To assess the diagnostic utility of Ziehl-Neelsen staining in lymphadenopathy cases, since it is commonly used method in the diagnosi...

Poor laboratory equipment and few human resources have made it difficult to implement microscopic diagnosis of pulmonary tuberculosis (TB) on a large scale basis worldwide. Three hundred sputum samples from patients in Cameroon were studied by using the CyScope®, a new light-emitting, diode-based, fluorescence microscope, to compare auramine-rhodamine fluorescence with the conventional Ziehl-Ne...

BACKGROUND We evaluated the Ziehl-Neelsen staining (ZNS) technique for the diagnosis of paragonimiasis in Laos and compared different modifications of the ZNS techniques. METHODOLOGY WE APPLIED THE FOLLOWING APPROACH: We (1) examined a paragonimiasis index case's sputum with wet film direct examination (WF) and ZNS; (2) re-examined stored ZNS slides from two provinces; (3) compared prospectiv...

The presence of acid fast bacilli in sputum specimen was investigated comparatively with Ziehl-Neelsen (ZN)and Kinyoun Cold staining (KC) in order to determine sensitivity in detecting Tuberculosis Bacilli (T.B.). This study was employed on patients with severe lower respiratory tract infection to compare between these two stains. Which revealed that Ziehl-Neelsen procedure gives better results...

Although inexpensive and easy to perform, the negative staining technique of Heine for the detection of Cryptosporidium spp. has been largely neglected. The lack of familiarity with the negative staining technique, the misconception that a phase-contrast microscope is indispensible and inferior results due to Köhler-illumination, are possible explanations for its low popularity. While the modif...