ten female lambs of 7-month-old were divided into two equal groups and raised under a helminth-free conditions. animals in group 1 were immunized two times by whole gut homogenate (wgh) of haemonchus contortus emulsified in freund’s adjuvant. in group 2 (control), animals were injected by phosphate buffered saline emulsified in the same adjuvant. animals were challenged by 10000 third-stage lar...

Ten female lambs of 7-month-old were divided into two equal groups and raised under a helminth-free conditions. Animals in group 1 were immunized two times by whole gut homogenate (WGH) of Haemonchus contortus emulsified in Freund’s adjuvant. In group 2 (control), animals were injected by phosphate buffered saline emulsified in the same adjuvant. Animals were challenged by 10000 third-stage lar...

The aim of this study was to synthesize a peptide prodrug of glucosamine (GlcN) with increased gut permeability through the gut peptide transporter 1 (PepT1). Glycine-Valine ester derivative of GlcN (GVG) was synthesised using solid phase synthesis followed by characterization and evaluation of its physicochemical and intestinal stability. In addition, GVG was evaluated for its ability to be bi...

Imatinib is an orally administered tyrosine kinase inhibitor which inhibits the Bcr-Abl protein-tyrosine kinase with high selectivity. Imatinib is rapidly absorbed from the gut, after oral intake and has an almost absolute bioavailability of 98%. The metabolism of imatinib is mediated by the cytochrome P450 (CYP) isoenzymes in the liver and gut wall. CGP74588 is a major active metabolite of ima...

imatinib is an orally administered tyrosine kinase inhibitor which inhibits the bcr-abl protein-tyrosine kinase with high selectivity. imatinib is rapidly absorbed from the gut, after oral intake and has an almost absolute bioavailability of 98%. the metabolism of imatinib is mediated by the cytochrome p450 (cyp) isoenzymes in the liver and gut wall. cgp74588 is a major active metabolite of ima...

Significant cholesterol synthesis occurs in gut mucosa of animals and humans. However, the role of gut synthesis in hypercholesterolemia and the effect of drugs on this function have not been defined. We obtained jejunal biopsies and bile samples from 21 Type II hypercholesterolemic subjects (mean serum cholesterol = 331 mg/dl) on a low fat diet after an over-night fast. Whole gut mucosal homog...

Several years ago we reported that formaldehyde could be isolated when dimethylaminoethanol, dimethylglycine, sarcosine, or methanol was incubated with a whole liver homogenate (1). Dimethylaminoethanol differed from the other substrates in that it required both the supernatant and sediment fractions of the homogenate to effect this biochemical oxidation. We have now prepared methyl-labeled dim...

Evaluation was performed of chemical compound contents and enzyme activities in the whole homogenate, its supernatant and sediment. Six rabbit livers were pulverized in liquid nitrogen and homogenized. After centrifugation, the contents of protein, haemoglobin, vitamin A, vitamin E, vitamin C, as well as the activities of cathepsin B, cathepsin D, superoxide dismutase, catalase, glutathione per...

While many current investigations involving oxidative phosphorylation are being carried out either with whole homogenates or with crude fractions derived therefrom, there are few in which (a) the results have been reported relative to the activity of the original homogenate, and (b) the fractions have been tested in various combinations. The need for such comparisons was shown in earlier result...